Anaerobic biobutanol production from black strap molasses using Clostridium acetobutylicum MTCC11274: Media engineering and kinetic analysis

Microbial reduction of black strap molasses (BSM) by Clostridium acetobutylicum MTCC 11,274 was performed for the production of biobutanol. The optimum fermentation conditions were predicted using one factor at a time (OFAT) method. The identification of significant parameters was performed using Plackett-Burman Design (PBD). Furthermore the fermentation conditions were optimized using central composite design (CCD). The kinetics of substrate utilization and product formation were investigated. Initial pH, yeast extract concentration (g/L) and total reducing sugar concentration (g/L) were found as significant parameters affecting butanol production using C. acetobutylicum MTCC11274. The maximum butanol production under optimal condition was 10.27 + 0.82 g/L after 24 h. The waste black strap molasses obtained from sugar industry could be used as promising substrate for the production of next generation biofuel.

Automated summary

In this study, microbial reduction of black strap molasses was performed using Clostridium acetobutylicum MTCC 11,274 to produce biobutanol. The significant parameters affecting butanol production were identified and optimal fermentation conditions were determined. The results showed that waste black strap molasses could be a promising substrate for the production of next generation biofuel.