The synergistic effect of rumen cellulolytic bacteria and activated carbon on thermophilic digestion of cornstalk

To explore the bioaugmentation of rumen cellulolytic bacteria (RCB) and activated carbon (AC) on thermophilic digestion of cornstalk, biochemical methane potential tests were carried out. Adding RCB or AC can improve methane production, while simultaneous existence of AC (10 g/L) and RCB (5%) obtained the best performance. The maximum cellulose degradation rate, methane production rate and methane yield were 66.92%, 32.2 L/ (kgVS.d), and 144.9 L/kgVS, which increased by 30.23%, 51.17%, and 20.35% compared with control group. The cellulolytic and fermentative bacteria (Hydrogenispora), syntrophic acetate-oxidizing bacteria (nor-ank_o_MBA03), and hydrogenotrophic Methanothermobacter were crucial for thermophilic digestion of cornstalk. The enhancement of AC was due to the enrichment of Hydrogenispora and Methanothermobacter, while RCB can increase the abundance of cellulolytic bacteria (Halocella and norank_o_M55-D21) and mixotrophic Meth-anosarcina. The synergetic effect of AC and RCB owing to the enriched cellulolytic bacteria, the enhanced syn-trophic acetate oxidation and the concentrated carbon metabolic flow to methane.

Automated summary

The addition of rumen cellulolytic bacteria and activated carbon can enhance methane production during thermophilic digestion of cornstalk, with the best performance achieved when both are present simultaneously. This improvement is attributed to the synergistic effect of the enriched bacteria working together.