Journal
BIORESOURCE TECHNOLOGY
Volume 337, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2021.125409
Keywords
Starch recovery; Protein recovery; Hydrothermal pretreatment; Biorefinery; Brewer's spent grain
Funding
- Swedish Agency for Economic and Regional Growth (Tillvadxtverket) through a European Regional Devel-opment Fund
- Godteborgs Nya Bryggeri AB
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This study successfully recovered a highly concentrated starch and protein stream from brewer's spent grain through hydrothermal pretreatment, and used it for fungal cultivation, resulting in pure fungal biomass with high protein content.
This study aimed at recovering a highly concentrated starch and protein stream from the brewer's spent grain (BSG). The effect of pretreatment temperature and retention time on the solubilization of starch and protein; and the generation of fermentation inhibitors were studied. Then, the application of recovered streams for fungal cultivation was evaluated using different edible fungi Aspergillus oryzae, Neurospora intermedia, and Rhizopus delemar. The hydrothermal pretreatment resulted in the highest solubilized starch concentration, 43 g/L, corresponding to 83% solubilization of initial BSG starch content. The highest protein concentration was 27 g/L (48% solubilization of initial BSG protein content). Cultivation with Neurospora intermedia on the recovered streams from the two best pretreatment conditions, 140 degrees C for 4 h and 180 degrees C for 30 min, resulted in pure fungal biomass with the highest protein content 59.62% and 50.42% w/w, respectively. Finally, a brewery biorefinery was proposed for the valorization of BSG.
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