Rapid initiation of methanogenesis in the anaerobic digestion of food waste by acclimatizing sludge with sulfidated nanoscale zerovalent iron

Although coupling of sulfidated nanoscale zero-valent iron (S-nZVI) into anaerobic digestion of food waste (FW) for improving methanogenesis has been reported, the specific role of S-nZVI during start-up process and its influence on subsequent methanogenesis and system stability remains unknown. In this study, S-nZVI was added into the unacclimatized sludge system to investigate its influence on microbial acclimatization and methanogenic performance. During acclimatization phase, CH4 production improved and VFAs transformation facilitated with the addition of S-nZVI. Furthermore, enzymatic activity analysis and electrochemical measurements presented direct evidence that electron transfer capacity of acclimatized sludge was significantly improved. S-nZVI favored the transition of microbial community to a robust and specialized population. During evaluation phase, acclimatized sludge still exhibited strong methanogenic ability, but the microbial community inevitably changed under the stress of FW. This research provides a novel perspective on initiating anaerobic digestion of FW for shorter start-up time and stronger methanogenesis.

Automated summary

The study found that the addition of S-nZVI improves methane production and VFAs transformation during the acclimatization phase, and enhances the electron transfer capacity of the microbial community. In the evaluation phase, the acclimatized sludge still maintains strong methanogenic ability, but the microbial community changes under the stress of food waste.