Comparative performance and reusability studies of lipases on syntheses of octyl esters with an economic approach

A suitable immobilized lipase for esters syntheses should be selected considering not only its cost. We evaluated five biocatalysts in syntheses of octyl caprylate, octyl caprate, and octyl laurate, in which conversions higher than 90% were achieved. Novozym(R) 435 and non-commercial preparations (including a dry fermented solid) were selected for short-term octyl laurate syntheses using different biocatalysts loadings. By increasing the biocatalyst's loading the lipase's reusability also raised, but without strict proportionality, which resulted in a convergence between the lowest biocatalyst loading and the lowest cost per batch. The use of a dry fermented solid was cost-effective, even using loadings as high as 20.0% wt/wt due to its low obtaining cost, although exhibiting low productiveness. The combination of biocatalyst's cost, esterification activity, stability, and reusability represents proper criteria for the choice. This kind of assessment may help to establish quantitative goals to improve or to develop new biocatalysts.

Automated summary

When selecting immobilized lipase for ester synthesis, factors such as cost, conversion rate, and reusability should be considered. Increasing the biocatalyst's loading can improve reusability of the lipase, but not in strict proportion, leading to a balance between the lowest biocatalyst loading and the lowest cost per batch.