Journal
BIOMEDICINE & PHARMACOTHERAPY
Volume 144, Issue -, Pages -Publisher
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.biopha.2021.112264
Keywords
Combretum hartmannianum; Traditional medicine; Tuberculosis; Ellagitannins; Flavonoids; Terpenoids
Funding
- Ministry of Higher Education
- University of Khartoum, Sudan
- Ekhaga Foundation, Stockholm, Sweden [2017-7]
- Swedish Cultural Foundation in Finland [159102]
- Helsinki University Library (HuLib) in Finland
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In Sudanese traditional medicine, extracts from the stem and root of Combretum hartmannianum are used to treat persistent cough, possibly related to tuberculosis. This study found that the ethyl acetate extract of the root showed strong growth inhibitory effects against Mycobacterium smegmatis, containing various polyphenols. Among these compounds, luteolin exhibited the best inhibition against M. smegmatis compared to other compounds like corilagin, ellagic acid, and gallic acid.
In Sudanese traditional medicine, decoctions, macerations, and tonics of the stem and root of Combretum hart-mannianum are used for the treatment of persistent cough, a symptom that could be related to tuberculosis (TB). To verify these traditional uses, extracts from the stem wood, stem bark, and roots of C. hartmannianum were screened for their growth inhibitory effects against Mycobacterium smegmatis ATCC 14468. Methanol Soxhlet and ethyl acetate extracts of the root gave the strongest effects (MIC 312.5 and 625 mu g/ml, respectively). HPLC-UV/DAD and UHPLC/QTOF-MS analysis of the ethyl acetate extract of the root led to the detection of 54 compounds, of which most were polyphenols and many characterized for the first time in C. hartmannianum. Among the major compounds were terflavin B and its two isomers, castalagin, corilagin, tellimagrandin I and its derivative, (S)-flavogallonic acid dilactone, punicalagin, and methyl-ellagic acid xylopyranoside. In addition, di-, tri- and tetragalloyl glucose, combregenin, terminolic acid, cordifoliside D, luteolin, and quercetin-3-O-galactoside-7-O-rhamnoside-(2 -> 1)-O-beta-D-arabinopyranoside were characterized. Luteolin gave better growth inhibition against M. smegmatis (MIC 250 mu g/ml) than corilagin, ellagic acid, and gallic acid (MIC 500-1000 mu g/ml). Our study justifies the use of C. hartmannianum in Sudanese folk medicine against prolonged cough that could be related to TB infection. This study demonstrates that C. hartmannianum should be explored further for new anti-TB drug scaffolds and antibiotic adjuvants.
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