Generation of ENSEMBL-based proteogenomics databases boosts the identification of non-canonical peptides

Article Biochemical Research Methods

Spritz: A Proteogenomic Database Engine

Anthony J. Cesnik et al.

Summary: Spritz is an open-source software tool developed for generating protein databases with sequence variations and PTMs annotations, enabling the identification and quantification of proteoforms by mass spectrometry. These sample-specific databases allow the identification of variant peptides, modified variant peptides, and variant proteoforms.

JOURNAL OF PROTEOME RESEARCH (2021)

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Article Cell Biology

Most non-canonical proteins uniquely populate the proteome or immunopeptidome

Maria Virginia Ruiz Cuevas et al.

Summary: By combining RNA sequencing, ribosome profiling, and mass spectrometry, the study reveals the significant contribution of non-canonical translation to the proteome and MHC class I immunopeptidome. It identifies a large number of cryptic proteins and shows their high efficiency in generating MHC-I peptides.

CELL REPORTS (2021)

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Article Biochemistry & Molecular Biology