Impact of amino acids on enhancing trimethyl chitosan as a nontoxic antiproliferative active biopolymer

This work evaluates the toxicity of trimethyl chitosan (TMC), which prevents its use as an antiproliferative active compound. We report our trial to enhance its safe application by chelating with various amino acids (phenylalanine, glycine and lysine). The safety evaluation was achieved by studying the behavior of TMC-amino acid chelates against two normal human cell lines (BJ1 and RPE1), in comparison with a negative control and doxrobicin (positive control) standard samples. The results obtained show that phenylalanine and lysine play an effective role in enhancing the safety of TMC against normal cell lines, together with providing high thermal stability. Phenylalanine is superior in synthesized anticancer agents. It had IC50 values against colon HCT116 and breast cancer MCF-7cancer cell lines of 68.7 and 51.4 mu g mL(-1) This compound is more acceptable than the previous reported rutin flavonoid as an anticancer agent against HCT116 cell lines. (c) 2021 Society of Chemical Industry and John Wiley & Sons, Ltd

Automated summary

Phenylalanine and lysine can enhance the safety of TMC against normal cell lines while providing high thermal stability. Phenylalanine shows superior performance in synthesizing anticancer agents.