Journal
BIOFOULING
Volume 38, Issue 2, Pages 131-146Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/08927014.2022.2028280
Keywords
Pseudomonas aeruginosa; biofilm inhibitor; LasR antagonists; quorum sensing; anti-biofilm peptides
Funding
- Ferdowsi University of Mashhad
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The novel peptides designed in this study have inhibitory effects on biofilm production and expression of related genes in Pseudomonas aeruginosa, exhibiting anti-biofilm effects at sub-inhibitory concentrations and inhibiting planktonic cell growth.
Pseudomonas aeruginosa is one of the most common biofilm-producing bacteria, often leading to long-term and chronic infections. The LasR regulator protein acts as the central regulator of the quorum sensing (QS) system and coordinates the expression of some virulence and biofilm genes. In this study, novel peptides (WSF, FASK, YDVD) were designed for binding to the domain of the transcriptional activator of the LasR protein and interfere with LasR in the QS system of P. aeruginosa. The effects of these peptides on biofilm production, expression of biofilm-related genes (AlgC, PslA, PelA), and growth of planktonic P. aeruginosa were investigated. All three peptides inhibited the growth of P. aeruginosa planktonic cells at 1600 mu g ml(-1) and exhibited anti-biofilm effects at sub-inhibitory concentrations (800 mu g ml(-1)). Measurements of the mRNA levels of biofilm-related genes at sub-inhibitory concentrations of the designed peptides showed a significant decrease.
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