4.3 Review

Structural foundations of sticholysin functionality

Journal

Publisher

ELSEVIER
DOI: 10.1016/j.bbapap.2021.140696

Keywords

actinoporins; structure-function relationship; lipid membranes

Funding

  1. Sigrid Juselius Foundation
  2. Jane and Aatos Erkko Foundation
  3. Magnus Ehrnrooth Foundation
  4. UCM-Banco Santander Grants [PR75/18-21561, PR87/19-22556, PR108/20-15]
  5. UCM-Banco Santander fellowship
  6. ISB/AA

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Actinoporins are a family of pore-forming toxins produced by sea anemones, which exert their activity by specifically recognizing sphingomyelin at their target membranes and killing cells. This review examines the structural features of actinoporins down to the residue level, focusing on the roles of specific amino acids in their function and fold. The membrane requirements for pore formation, the effect of lipid composition, and the process of pore formation are also discussed in light of the latest findings in the field.
Actinoporins constitute a family of a pore-forming toxins produced by sea anemones. The soluble fold of these proteins consists of a beta-sandwich flanked by two alpha-helices. Actinoporins exert their activity by specifically recognizing sphingomyelin at their target membranes. Once there, they penetrate the membrane with their N-terminal alpha-helices, a process that leads to the formation of cation-selective pores. These pores kill the target cells by provoking an osmotic shock on them. In this review, we examine the role and relevance of the structural features of actinoporins, down to the residue level. We look at the specific amino acids that play significant roles in the function of actinoporins and their fold. Particular emphasis is given to those residues that display a high degree of conservation across the actinoporin sequences known to date. In light of the latest findings in the field, the membrane requirements for pore formation, the effect of lipid composition, and the process of pore formation are also discussed.

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