Journal
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
Volume 1869, Issue 10, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.bbapap.2021.140684
Keywords
Galectin; Carbohydrate recognition; Site-directed mutagenesis
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This study elucidated the key residues for carbohydrate specificity of Xenopus laevis skin galectin xgalectin-Va through site-directed mutagenesis, leading to a successful change in carbohydrate selectivity. The mutation of two amino acids greatly enhanced the binding ability to N-acetyllactosamine and conferred significant T-cell growth inhibition activity. The loop substitution also successfully altered the carbohydrate selectivity of xgalectin-Va and xgalectin-Ib.
The galectin family is a representative soluble lectin group, which is responsible for the modulation of various cell functions. Although the carbohydrate-binding specificity of galectins has been well-studied, the relationship between protein structure and specificity remains to be elucidated. We previously reported the characteristics of a Xenopus laevis skin galectin, xgalectin-Va, which had diverged from galectin-1. The carbohydrate selectivity of xgalectin-Va was different from that of human galectin-1 and xgalectin-Ib (a Xenopus laevis galectin-1 homolog). In this study, we clarified the key residues for this selectivity by site-directed mutagenesis. Substitution of two amino acids of xgalectin-Va, Va156Gly/Lys76Arg, greatly enhanced the binding ability to N-acetyllactosamine and conferred significant T-cell growth inhibition activity, although the wild type had no activity. These two residues, Gly54 and Arg74 in galectin-1, would cooperatively contribute to the N-acetyllactosamine recognition. The loop region between the S4 and S5 beta-strands was involved in the binding to the TF-antigen disaccharide. The loop substitution successfully changed the carbohydrate selectivity of xgalectin-Va and xgalectin-Ib.
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