Journal
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
Volume 1867, Issue 11, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.bbadis.2021.166219
Keywords
CRISPR/Cas9; SIRT7; Knockout; Melanin; Melanoma cells
Funding
- Basic Science Research Program
- National Research Foundation ofKorea (NRF) - Ministry of Education [2017R1D1A3B06028000]
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Melanin is a key pigment in skin and hair, with deficiency leading to various disorders. Enhancing melanin production at a genetic level could offer an effective and permanent solution for these disorders, with the gene SIRT7 potentially playing a crucial role in this process.
Melanin is a prominent pigment of skin and hair, and its deficiency can cause various disorders such as hair graying and albinism. The improvement of melanin production at a genetic level could offer an effective and permanent solution. Recently, SIRT7 has evoked an interest in the study of hair follicle stem cells, but its role in melanin synthesis remains unclear. In the present study, we have first successfully developed SIRT7 gene KO melanoma cells using the CRISPR/Cas9 system. It was found that the SIRT7 gene KO enhanced melanin production in melanoma cells. To validate the role of SIRT7 in melanin production, RT-PCR, western blot, and immunofluorescence staining assays were performed. The expression levels of melanin-producing genes and proteins (MITF, TRP1, TRP-2, TYR, TH) were significantly increased in SIRT7 gene KO cells compared to normal cells. In addition, melanin production was increased in KO cells higher than in normal cells through the image analysis. All these results suggest that SIRT7 could play an essential role in regulating melanin production, providing an alternative drug target to treat pigmentary disorders.
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