Journal
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
Volume 1866, Issue 10, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.bbalip.2021.158992
Keywords
Lipid droplet-anchored mitochondria; BAT; Comparative proteomics; FA beta-oxidation
Funding
- National Key Research and Development Program of China [2018YFA0800700, 2018YFA0800900, 2016YFA0500100]
- National Natural Science Foundation of China [91954108, 91857201, U1702288]
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Brown adipose tissue (BAT) contains lipid droplet-anchored mitochondria (LDAM) which are functional across different temperatures, with comparative proteomics analysis revealing differential protein expressions between LDAM and cytoplasmic mitochondria (CM) at different temperatures, indicating a preorganized and functional organelle complex.
Brown adipose tissue (BAT) is specialized for uncoupled heat production through mitochondrion fueled majorly from fatty acids (FAs) of lipid droplets (LDs). How the interaction between the two organelles contributes the generation of heat remains elusive. Here, we report that LD-anchored mitochondria (LDAM) were observed in the BAT of mice raised at three different temperatures, 30 degrees C, 23 degrees C, and 6 degrees C. The biochemical analyses including Western blotting of electron transport chain subunits showed that LDAM were functional. Comparative proteomics analysis was conducted, which revealed differential expressions of proteins between LDAM and cytoplasmic mitochondria (CM) at different temperatures. Higher expressions of proteins at low temperature were observed for i) FA beta-oxidation in LDAM including FA synthesis and uncoupling, ii) pseudo-futile cycle in CM, and iii) two shuttle systems: glycerol 3-phosphate in both CM and LDAM and citrate malate in CM. Together, these results suggest that LDs and LDAM form a preorganized and functional organelle complex that permits the rapid response to cold.
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