Journal
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
Volume 1864, Issue 2, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.bbamem.2021.183828
Keywords
Anion transport; Hydrogen bonding; Thiosemicarbazones; Switchable receptors
Categories
Funding
- University of Sydney
- Australian Research Council [DP200100453]
- Australian Research Council [DP200100453] Funding Source: Australian Research Council
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Phenylthiosemicarbazones (PTSCs) are proton-coupled anion transporters that exhibit pH-switchable behavior. This study demonstrates that protonated PTSCs are highly selective for halides over oxyanions in membrane transport, and the pH-dependent chloride/nitrate exchange is due to the rate-limiting nitrate transport process at neutral pH.
Phenylthiosemicarbazones (PTSCs) are proton-coupled anion transporters with pH-switchable behaviour known to be regulated by an imine protonation equilibrium. Previously, chloride/nitrate exchange by PTSCs was found to be inactive at pH 7.2 due to locking of the thiourea anion binding site by an intramolecular hydrogen bond, and switched ON upon imine protonation at pH 4.5. The rate-determining process of the pH switch, however, was not examined. We here develop a new series of PTSCs and demonstrate their conformational behaviour by Xray crystallographic analysis and pH-switchable anion transport properties by liposomal assays. We report the surprising finding that the protonated PTSCs are extremely selective for halides over oxyanions in membrane transport. Owing to the high chloride over nitrate selectivity, the pH-dependent chloride/nitrate exchange of PTSCs originates from the rate-limiting nitrate transport process being inhibited at neutral pH.
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