Directed evolution approaches for optogenetic tool development

Photoswitchable proteins enable specific molecular events occurring in complex biological settings to be probed in a rapid and reversible fashion. Recent progress in the development of photoswitchable proteins as components of optogenetic tools has been greatly facilitated by directed evolution approaches in vitro, in bacteria, or in yeast. We review these developments and suggest future directions for this rapidly advancing field.

Automated summary

Photoswitchable proteins play a crucial role in optogenetics, allowing specific molecular events in complex biological settings to be probed. Recent advancements in this field, facilitated by directed evolution approaches in vitro, bacteria, or yeast, have paved the way for future developments.