Viscoelastic properties of epithelial cells

Epithelial cells form tight barriers that line both the outer and inner surfaces of organs and cavities and therefore face diverse environmental challenges. The response to these challenges relies on the cells' dynamic viscoelastic properties, playing a pivotal role in many biological processes such as adhesion, growth, differentiation, and motility. Therefore, the cells usually adapt their viscoelastic properties to mirror the environment that determines their fate and vitality. Albeit not a high-throughput method, atomic force microscopy is still among the dominating methods to study the mechanical properties of adherent cells since it offers a broad range of forces from Piconewtons to Micronewtons at biologically significant time scales. Here, some recent work of deformation studies on epithelial cells is reviewed with a focus on viscoelastic models suitable to describe force cycle measurements congruent with the architecture of the actin cytoskeleton. The prominent role of the cortex in the cell's response to external forces is discussed also in the context of isolated cortex extracts on porous surfaces.

Automated summary

Epithelial cells form tight barriers on the outer and inner surfaces of organs and cavities, facing diverse environmental challenges that rely on their dynamic viscoelastic properties. Studying mechanical properties through atomic force microscopy and viscoelastic models can provide insights into cell responses to external forces. The cortex plays a significant role in the response of cells to external forces, as discussed in recent deformation studies.