Journal
BIOCHEMICAL ENGINEERING JOURNAL
Volume 176, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.bej.2021.108181
Keywords
Valproic acid; Epilepsy; Biosensor; Anti-epileptic; Potentiometry
Funding
- Tokat Gaziosmanpasa University, Scientific Research Projects Commission [2019/89]
- Scientific and Technological Research Council of Turkey (TUBITAK)-BIDEB 2211/A
- Council of Higher Education (YOK) 100/2000 National Scholarship Programs for PhD students
- TUBITAK
- YOK
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The potentiometric PVC membrane-based biosensor developed for the determination of valproic acid in human blood samples showed good accuracy, low detection limit, and high selectivity for anti-epileptic drugs.
A potentiometric poly (vinyl chloride) (PVC) membrane-based biosensor has been developed for the determination of the anti-epileptic drug valproic acid in human blood samples. The valpmate-selective biosensor worked well over a wide concentration range from 1.0 x 10(-6) to 1.0 x 10(-1) mol L-1 with Nernstian slope of 59.0 + 3.6 mV/decade. The biosensor exhibited a lower detection limit of 9.75 x 10(-7) mol L-1. The developed valproate-selective biosensor showed very good selectivity for anti-epileptic drugs such as carbamazepine, levetiracetam and phenytoin, and for some ions present in human blood. The biosensor displayed a fast response time of less than 10 s, good reusability and stability, and operated in the wide pH range of 4.0-11.0. The morphology of the developed PVC membrane biosensor was analyzed by scanning electron microscopy. Finally, the determination of valproic acid in human blood samples was carried out using the standard addition method with very high recoveries.
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