Related references
Note: Only part of the references are listed.The Fold-Illuminator: A low-cost, portable, and disposable incubator-illuminator device
Logan R. Burrington et al.
SYNTHETIC AND SYSTEMS BIOTECHNOLOGY (2021)
An enhanced assay to characterize anti-CRISPR proteins using a cell-free transcription-translation system
Katharina G. Wandera et al.
METHODS (2020)
Synthetic Biology Enables Programmable Cell-Based Biosensors
Maggie Hicks et al.
CHEMPHYSCHEM (2020)
Cell-free gene expression: an expanded repertoire of applications
Adam D. Silverman et al.
NATURE REVIEWS GENETICS (2020)
Engineering Cell-Free Protein Synthesis for High-Yield Production and Human Serum Activity Assessment of Asparaginase: Toward On-Demand Treatment of Acute Lymphoblastic Leukemia
J. Porter Hunt et al.
BIOTECHNOLOGY JOURNAL (2020)
Unlocking Applications of Cell-Free Biotechnology through Enhanced Shelf Life and Productivity of E. coli Extracts
Nicole E. Gregorio et al.
ACS SYNTHETIC BIOLOGY (2020)
Design of a Transcriptional Biosensor for the Portable, On-Demand Detection of Cyanuric Acid
Xiangyang Liu et al.
ACS SYNTHETIC BIOLOGY (2020)
Active Analyte Import Improves the Dynamic Range and Sensitivity of a Vitamin B12 Biosensor
Monica P. McNerney et al.
ACS SYNTHETIC BIOLOGY (2020)
Design and Optimization of a Cell-Free Atrazine Biosensor
Adam D. Silverman et al.
ACS SYNTHETIC BIOLOGY (2020)
Point-of-Use Detection of Environmental Fluoride via a Cell-Free Riboswitch-Based Biosensor
Walter Thavarajah et al.
ACS SYNTHETIC BIOLOGY (2020)
Sequential Glycosylation of Proteins with Substrate-Specific N-Glycosyltransferases
Liang Lin et al.
ACS CENTRAL SCIENCE (2020)
O2-Tuned Protein Synthesis Machinery in Escherichia coli-Based Cell-Free System
Xiaomei Lin et al.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY (2020)
Total in vitro biosynthesis of the nonribosomal macrolactone peptide valinomycin
Lei Zhuang et al.
METABOLIC ENGINEERING (2020)
Cell-free biosensors for rapid detection of water contaminants
Jaeyoung K. Jung et al.
NATURE BIOTECHNOLOGY (2020)
In vitro prototyping and rapid optimization of biosynthetic enzymes for cell design
Ashty S. Karim et al.
NATURE CHEMICAL BIOLOGY (2020)
Activation of Energy Metabolism through Growth Media Reformulation Enables a 24-Hour Workflow for Cell-Free Expression
Max Z. Levine et al.
ACS SYNTHETIC BIOLOGY (2020)
Engineering microbial diagnostics and therapeutics with smart control
Matthew B. Amrofell et al.
CURRENT OPINION IN BIOTECHNOLOGY (2020)
In vitro reconstitution of functional small ribosomal subunit assembly for comprehensive analysis of ribosomal elements in E. coli
Masaru Shimojo et al.
COMMUNICATIONS BIOLOGY (2020)
Endotoxin-Free E-coli-Based Cell-Free Protein Synthesis: Pre-Expression Endotoxin Removal Approaches for on-Demand Cancer Therapeutic Production
Kristen M. Wilding et al.
BIOTECHNOLOGY JOURNAL (2019)
Endotoxin-Free E-coli-Based Cell-Free Protein Synthesis: Pre-Expression Endotoxin Removal Approaches for on-Demand Cancer Therapeutic Production
Kristen M. Wilding et al.
BIOTECHNOLOGY JOURNAL (2019)
Interfacing a Personal Glucose Meter with Cell-Free Protein Synthesis for Rapid Analysis of Amino Acids
Yeon-Jae Jang et al.
ANALYTICAL CHEMISTRY (2019)
Synthetic biology and metabolic engineering of actinomycetes for natural product discovery
Emilia Palazzotto et al.
BIOTECHNOLOGY ADVANCES (2019)
Tandem Cell-Free Protein Synthesis as a Tool for Rapid Screening of Optimal Molecular Chaperones
Hyeon-Jung Yang et al.
BIOTECHNOLOGY JOURNAL (2019)
Cell-Free Protein Synthesis Using S30 Extracts from Escherichia coli RFzero Strains for Efficient Incorporation of Non-Natural Amino Acids into Proteins
Jiro Adachi et al.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2019)
A paper-based, cell-free biosensor system for the detection of heavy metals and date rape drugs
Alexander Graewe et al.
PLOS ONE (2019)
Osmolyte-Enhanced Protein Synthesis Activity of a Reconstituted Translation System
Yoshiki Moriizumi et al.
ACS SYNTHETIC BIOLOGY (2019)
BioBits Health: Classroom Activities Exploring Engineering, Biology, and Human Health with Fluorescent Readouts
Jessica C. Stark et al.
ACS SYNTHETIC BIOLOGY (2019)
Exploration of the Tolerance Ability of a Cell-Free Biosynthesis System to Toxic Substances
Peng Zhang et al.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY (2019)
Plug-and-play metabolic transducers expand the chemical detection space of cell-free biosensors
Peter L. Voyvodic et al.
NATURE COMMUNICATIONS (2019)
Gene Expression in on-Chip Membrane-Bound Artificial Cells
Ziane Izri et al.
ACS SYNTHETIC BIOLOGY (2019)
An Adaptive Synthetic Cell Based on Mechanosensing, Biosensing, and Inducible Gene Circuits
Jonathan Garamella et al.
ACS SYNTHETIC BIOLOGY (2019)
Metabolic Profiling of Escherichia coli-Based Cell-Free Expression Systems for Process Optimization
April M. Miguez et al.
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH (2019)
Point-of-care biomarker quantification enabled by sample-specific calibration
Monica P. McNerney et al.
SCIENCE ADVANCES (2019)
Thermostable lyoprotectant-enhanced cell-free protein synthesis for on-demand endotoxin-free therapeutic production
Kristen M. Wilding et al.
NEW BIOTECHNOLOGY (2019)
A cell-free biosynthesis platform for modular construction of protein glycosylation pathways
Weston Kightlinger et al.
NATURE COMMUNICATIONS (2019)
In Vitro Use of Cellular Synthetic Machinery for Biosensing Applications
Kyung-Ho Lee et al.
FRONTIERS IN PHARMACOLOGY (2019)
Cell-free protein synthesis: Recent advances in bacterial extract sources and expanded applications
Wan-Qiu Liu et al.
BIOCHEMICAL ENGINEERING JOURNAL (2019)
Distinct timescales of RNA regulators enable the construction of a genetic pulse generator
Alexandra Westbrook et al.
BIOTECHNOLOGY AND BIOENGINEERING (2019)
Rapid prototyping of proteins: Mail order gene fragments to assayable proteins within 24 hours
Jared Lynn Dopp et al.
BIOTECHNOLOGY AND BIOENGINEERING (2019)
Cascading Amplification of Immunoassay Signal by Cell-Free Expression of Firefly Luciferase from Detection Antibody-Conjugated DNA in an Escherichia coli Extract
Ju-Young Byun et al.
ACS SENSORS (2019)
Dynamic and tunable metabolite control for robust minimal-equipment assessment of serum zinc
Monica P. McNerney et al.
NATURE COMMUNICATIONS (2019)
In vitro implementation of robust gene regulation in a synthetic biomolecular integral controller
Deepak K. Agrawal et al.
NATURE COMMUNICATIONS (2019)
Towards On-Demand E. coli-Based Cell-Free Protein Synthesis of Tissue Plasminogen Activator
Seung-Ook Yang et al.
METHODS AND PROTOCOLS (2019)
An educational module to explore CRISPR technologies with a cell-free transcription-translation system
Daphne Collias et al.
SYNTHETIC BIOLOGY (2019)
Cell-free biosynthesis of limonene using enzyme-enriched Escherichia coli lysates
Quentin M. Dudley et al.
SYNTHETIC BIOLOGY (2019)
A Crude Extract Preparation and Optimization from a Genomically Engineered Escherichia coli for the Cell-Free Protein Synthesis System: Practical Laboratory Guideline
Jeehye Kim et al.
METHODS AND PROTOCOLS (2019)
Methods to reduce variability in E. Coli-based cell-free protein expression experiments
Jared L. Dopp et al.
SYNTHETIC AND SYSTEMS BIOTECHNOLOGY (2019)
Streamlining the preparation of endotoxin-free ClearColi cell extract with autoinduction media for cell-free protein synthesis of the therapeutic protein crisantaspase
J. Porter Hunt et al.
SYNTHETIC AND SYSTEMS BIOTECHNOLOGY (2019)
Dynamic Control of Aptamer-Ligand Activity Using Strand Displacement Reactions
Jonathan Lloyd et al.
ACS SYNTHETIC BIOLOGY (2018)
Polyvinylsulfonic acid: A Low-cost RNase inhibitor for enhanced RNA preservation and cell-free protein translation
Conner C. Earl et al.
BIOENGINEERED (2018)
Perspective: Solidifying the impact of cell-free synthetic biology through lyophilization
Keith Pardee
BIOCHEMICAL ENGINEERING JOURNAL (2018)
The emerging impact of cell-free chemical biosynthesis
Kristen M. Wilding et al.
CURRENT OPINION IN BIOTECHNOLOGY (2018)
Rapid and Scalable Characterization of CRISPR Technologies Using an E. coli Cell-Free Transcription-Translation System
Ryan Marshall et al.
MOLECULAR CELL (2018)
Design of glycosylation sites by rapid synthesis and analysis of glycosyltransferases
Weston Kightlinger et al.
NATURE CHEMICAL BIOLOGY (2018)
Cell-free synthesis of functional antibody fragments to provide a structural basis for antibody-antigen interaction
Takayoshi Matsuda et al.
PLOS ONE (2018)
Rapid acquisition and model-based analysis of cell-free transcription-translation reactions from nonmodel bacteria
Simon J. Moore et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2018)
Phosphorylated and non-phosphorylated HCK kinase domains produced by cell-free protein expression
Kazushige Katsura et al.
PROTEIN EXPRESSION AND PURIFICATION (2018)
Biosensing estrogenic endocrine disruptors in human blood and urine: A RAPID cell-free protein synthesis approach
Amin S. M. Salehi et al.
TOXICOLOGY AND APPLIED PHARMACOLOGY (2018)
Cell-free prediction of protein expression costs for growing cells
Olivier Borkowski et al.
NATURE COMMUNICATIONS (2018)
Single-pot glycoprotein biosynthesis using a cell-free transcription-translation system enriched with glycosylation machinery
Thapakorn Jaroentomeechai et al.
NATURE COMMUNICATIONS (2018)
Cell-free protein synthesis from genomically recoded bacteria enables multisite incorporation of noncanonical amino acids
Rey W. Martin et al.
NATURE COMMUNICATIONS (2018)
Cell-free protein synthesis as a novel tool for directed glycoengineering of active erythropoietin
Anne Zemella et al.
SCIENTIFIC REPORTS (2018)
Robustness of a Reconstituted Escherichia coli Protein Translation System Analyzed by Computational Modeling
Tomoaki Matsuura et al.
ACS SYNTHETIC BIOLOGY (2018)
Sequence Specific Modeling of E. coli Cell-Free Protein Synthesis
Michael Vilkhovoy et al.
ACS SYNTHETIC BIOLOGY (2018)
Point-of-care production of therapeutic proteins of good-manufacturing-practice quality
Rajani Adiga et al.
NATURE BIOMEDICAL ENGINEERING (2018)
Reconstitution of 30S ribosomal subunits in vitro using ribosome biogenesis factors
Daichi Tamaru et al.
RNA (2018)
A low-cost paper-based synthetic biology platform for analyzing gut microbiota and host biomarkers
Melissa K. Takahashi et al.
NATURE COMMUNICATIONS (2018)
BioBits™ Explorer: A modular synthetic biology education kit
Ally Huang et al.
SCIENCE ADVANCES (2018)
Cell-free synthetic biology for in vitro biosynthesis of pharmaceutical natural products
Jian Li et al.
SYNTHETIC AND SYSTEMS BIOTECHNOLOGY (2018)
G-Protein Coupled Receptor Protein Synthesis on a Lipid Bilayer Using a Reconstituted Cell-Free Protein Synthesis System
Belay Gessesse et al.
LIFE-BASEL (2018)
Cell-free synthesis of stable isotope-labeled internal standards for targeted quantitative proteomics
Ryohei Narumi et al.
SYNTHETIC AND SYSTEMS BIOTECHNOLOGY (2018)
Low-cost detection of norovirus using paper-based cell-free systems and synbody-based viral enrichment
Duo Ma et al.
SYNTHETIC BIOLOGY (2018)
Engineering DNA nanotubes for resilience in an E. coli TXTL system
Melissa A. Klocke et al.
SYNTHETIC BIOLOGY (2018)
Models for Cell-Free Synthetic Biology: Make Prototyping Easier, Better, and Faster
Mathilde Koch et al.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY (2018)
Antibiotics in the clinical pipeline at the end of 2015
Mark S. Butler et al.
JOURNAL OF ANTIBIOTICS (2017)
In Vitro Reconstruction of Nonribosomal Peptide Biosynthesis Directly from DNA Using Cell-Free Protein Synthesis
Anthony W. Goering et al.
ACS SYNTHETIC BIOLOGY (2017)
The growing impact of lyophilized cell-free protein expression systems
J. Porter Hunt et al.
BIOENGINEERED (2017)
After Theranos
Emily Waltz
NATURE BIOTECHNOLOGY (2017)
Production of virus-like particles for vaccines
J. Fuenmayor et al.
NEW BIOTECHNOLOGY (2017)
Reaction dynamics analysis of a reconstituted Escherichia coli protein translation system by computational modeling
Tomoaki Matsuura et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2017)
Synchrony and pattern formation of coupled genetic oscillators on a chip of artificial cells
Alexandra M. Tayar et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2017)
Rapid and Scalable Preparation of Bacterial Lysates for Cell-Free Gene Expression
Andriy Didovyk et al.
ACS SYNTHETIC BIOLOGY (2017)
A Cell-Free Biosensor for Detecting Quorum Sensing Molecules in P. aeruginosa-Infected Respiratory Samples
Ke Yan Wen et al.
ACS SYNTHETIC BIOLOGY (2017)
Cell free protein synthesis: a viable option for stratified medicines manufacturing?
Olotu W. Ogonah et al.
CURRENT OPINION IN CHEMICAL ENGINEERING (2017)
Immunoscreening of Plasmodium falciparum proteins expressed in a wheat germ cell-free system reveals a novel malaria vaccine candidate
Masayuki Morita et al.
SCIENTIFIC REPORTS (2017)
High-yield production of difficult-to-express proteins in a continuous exchange cell-free system based on CHO cell lysates
Lena Thoring et al.
SCIENTIFIC REPORTS (2017)
Proteomics-Based Tools for Evaluation of Cell-Free Protein Synthesis
Gregory B. Hurst et al.
ANALYTICAL CHEMISTRY (2017)
Complementary Cell-Free Translational Assay for Quantification of Amino Acids
Yeon-Jae Jang et al.
ANALYTICAL CHEMISTRY (2017)
Rapid in vitro screening for the location-dependent effects of unnatural amino acids on protein expression and activity
Song-Min Schinn et al.
BIOTECHNOLOGY AND BIOENGINEERING (2017)
Short DNA Containing χ Sites Enhances DNA Stability and Gene Expression in E-coli Cell-Free Transcription-Translation Systems
Ryan Marshall et al.
BIOTECHNOLOGY AND BIOENGINEERING (2017)
Cross-Reactive Synbody Affinity Ligands for Capturing Diverse Noroviruses
Nidhi Gupta et al.
ANALYTICAL CHEMISTRY (2017)
Cell-sized mechanosensitive and biosensing compartment programmed with DNA
Sagardip Majumder et al.
CHEMICAL COMMUNICATIONS (2017)
Modules for in vitro metabolic engineering: Pathway assembly for bio-based production of value-added chemicals
Hironori Taniguchi et al.
SYNTHETIC AND SYSTEMS BIOTECHNOLOGY (2017)
Translation System Engineering in Escherichia coli Enhances Non-Canonical Amino Acid Incorporation into Proteins
Rui Gan et al.
BIOTECHNOLOGY AND BIOENGINEERING (2017)
Cell-Free Protein Synthesis Approach to Biosensing hTR(ss)-Specific Endocrine Disruptors
Amin S. M. Salehi et al.
ANALYTICAL CHEMISTRY (2017)
Virus-like particles: Next-generation nanoparticles for targeted therapeutic delivery
Marcus J. Rohovie et al.
BIOENGINEERING & TRANSLATIONAL MEDICINE (2017)
Cell-free synthetic biology: Engineering in an open world
Yuan Lu
SYNTHETIC AND SYSTEMS BIOTECHNOLOGY (2017)
Cell-free protein synthesis of a cytotoxic cancer therapeutic: Onconase production and a just-add-water cell-free system
Amin S. M. Salehi et al.
BIOTECHNOLOGY JOURNAL (2016)
A cell-free expression and purification process for rapid production of protein biologics
Challise J. Sullivan et al.
BIOTECHNOLOGY JOURNAL (2016)
Cell-free protein synthesis enables high yielding synthesis of an active multicopper oxidase
Jian Li et al.
BIOTECHNOLOGY JOURNAL (2016)
Toward Microfluidic Reactors for Cell-Free Protein Synthesis at the Point-of-Care
Andrea C. Timm et al.
SMALL (2016)
Cell-free protein synthesis of a cytotoxic cancer therapeutic: Onconase production and a just-add-water cell-free system
Amin S. M. Salehi et al.
BIOTECHNOLOGY JOURNAL (2016)
A cell-free expression and purification process for rapid production of protein biologics
Challise J. Sullivan et al.
BIOTECHNOLOGY JOURNAL (2016)
Cell-free protein synthesis enables high yielding synthesis of an active multicopper oxidase
Jian Li et al.
BIOTECHNOLOGY JOURNAL (2016)
Biotechnological production of limonene in microorganisms
Esmer Jongedijk et al.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2016)
Cell-Free Production and Streamlined Assay of Cytosol-Penetrating Antibodies
Seung Eui Min et al.
BIOTECHNOLOGY AND BIOENGINEERING (2016)
Portable, On-Demand Biomolecular Manufacturing
Keith Pardee et al.
CELL (2016)
Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components
Keith Pardee et al.
CELL (2016)
Genetic Code Expansion by Degeneracy Reprogramming of Arginyl Codons
Ki Baek Lee et al.
CHEMBIOCHEM (2016)
Cell-free compartmentalized protein synthesis inside double emulsion templated liposomes with in vitro synthesized and assembled ribosomes
Filippo Caschera et al.
CHEMICAL COMMUNICATIONS (2016)
Enhanced production of unnatural amino acid-containing proteins in a cell-free protein synthesis system
Kyung-Ho Lee et al.
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY (2016)
Natural Products as Sources of New Drugs from 1981 to 2014
David J. Newman et al.
JOURNAL OF NATURAL PRODUCTS (2016)
A cell-free framework for rapid biosynthetic pathway prototyping and enzyme discovery
Ashty S. Karim et al.
METABOLIC ENGINEERING (2016)
Recombinant pharmaceuticals from microbial cells: a 2015 update
Laura Sanchez-Garcia et al.
MICROBIAL CELL FACTORIES (2016)
Next generation sequencing: implications in personalized medicine and pharmacogenomics
Bahareh Rabbani et al.
MOLECULAR BIOSYSTEMS (2016)
Protein synthesis directly from PCR: progress and applications of cell-free protein synthesis with linear DNA
Song-Min Schinn et al.
NEW BIOTECHNOLOGY (2016)
Cell-Free Synthetic Biology: Engineering Beyond the Cell
Jessica G. Perez et al.
COLD SPRING HARBOR PERSPECTIVES IN BIOLOGY (2016)
Cell-Free Mixing of Escherichia coli Crude Extracts to Prototype and Rationally Engineer High-Titer Mevalonate Synthesis
Quentin M. Dudley et al.
ACS SYNTHETIC BIOLOGY (2016)
Residue-specific Incorporation of Noncanonical Amino Acids into Model Proteins Using an Escherichia coli Cell-free Transcription-translation System
Emanuel G. Worst et al.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS (2016)
Functional properties of flagellin as a stimulator of innate immunity
Yuan Lu et al.
SCIENTIFIC REPORTS (2016)
Cell-free methods to produce structurally intact mammalian membrane proteins
Takehiro Shinoda et al.
SCIENTIFIC REPORTS (2016)
Integration of a Reconstituted Cell-free Protein-synthesis System on a Glass Microchip
Yo Tanaka et al.
ANALYTICAL SCIENCES (2015)
Cell-free expression with the toxic amino acid canavanine
Emanuel G. Worst et al.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS (2015)
Genetically expanded cell-free protein synthesis using endogenous pyrrolysyl orthogonal translation system
Yonatan Chemla et al.
BIOTECHNOLOGY AND BIOENGINEERING (2015)
Engineering Thermal Properties of Elastin-like Polypeptides by Incorporation of Unnatural Amino Acids in a Cell-free Protein Synthesis System
Christy Catherine et al.
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING (2015)
A Simplified and Robust Protocol for Immunoglobulin Expression in Escherichia coli Cell-Free Protein Synthesis Systems
Qi Cai et al.
BIOTECHNOLOGY PROGRESS (2015)
Creating a Completely Cell-free System for Protein Synthesis
Mark Thomas Smith et al.
BIOTECHNOLOGY PROGRESS (2015)
Improving Cell-Free Protein Synthesis through Genome Engineering of Escherichia coli Lacking Release Factor 1
Seok Hoon Hong et al.
CHEMBIOCHEM (2015)
Enhanced Enzyme Stability Through Site-Directed Covalent Immobilization
Jeffrey Chun Yu Wu et al.
JOURNAL OF BIOTECHNOLOGY (2015)
A cost-effective polyphosphate-based metabolism fuels an all E. coli cell-free expression system
Filippo Caschera et al.
METABOLIC ENGINEERING (2015)
Principal component analysis of proteomics (PCAP) as a tool to direct metabolic engineering
Jorge Alonso-Gutierrez et al.
METABOLIC ENGINEERING (2015)
Assessing sequence plasticity of a virus-like nanoparticle by evolution toward a versatile scaffold for vaccines and drug delivery
Yuan Lu et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2015)
pH responsive granulocyte colony-stimulating factor variants with implications for treating Alzheimer's disease and other central nervous system disorders
Pete Heinzelman et al.
PROTEIN ENGINEERING DESIGN & SELECTION (2015)
Robust production of recombinant phosphoproteins using cell-free protein synthesis
Javin P. Oza et al.
NATURE COMMUNICATIONS (2015)
Content of intrinsic disorder influences the outcome of cell-free protein synthesis
Alexander A. Tokmakov et al.
SCIENTIFIC REPORTS (2015)
High-throughput preparation methods of crude extract for robust cell-free protein synthesis
Yong-Chan Kwon et al.
SCIENTIFIC REPORTS (2015)
Cell-free metabolic engineering: Biomanufacturing beyond the cell
Quentin M. Dudley et al.
BIOTECHNOLOGY JOURNAL (2015)
Production of bispecific antibodies in knobs-into-holes using a cell-free expression system
Yiren Xu et al.
MABS (2015)
Clinical Application of Asparaginase Activity Levels Following Treatment With Pegaspargase
Archie Bleyer et al.
PEDIATRIC BLOOD & CANCER (2015)
Cell-Free Expression and In Situ Immobilization of Parasite Proteins from Clonorchis sinensis for Rapid Identification of Antigenic Candidates
Christy Catherine et al.
PLOS ONE (2015)
Rapidly Characterizing the Fast Dynamics of RNA Genetic Circuitry with Cell-Free Transcription Translation (TX-TL) Systems
Melissa K. Takahashi et al.
ACS SYNTHETIC BIOLOGY (2015)
Detoxifying Escherichia coli for endotoxin-free production of recombinant proteins
Uwe Mamat et al.
Microbial Cell Factories (2015)
Production of Site-Specific Antibody-Drug Conjugates Using Optimized Non-Natural Amino Acids in a Cell-Free Expression System
Erik S. Zimmerman et al.
BIOCONJUGATE CHEMISTRY (2014)
Translational incorporation of multiple unnatural amino acids in a cell-free protein synthesis system
Su-Jin Oh et al.
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING (2014)
Engineering the productivity of recombinant Escherichia coli for limonene formation from glycerol in minimal media
Christian Willrodt et al.
BIOTECHNOLOGY JOURNAL (2014)
Toehold Switches: De-Novo-Designed Regulators of Gene Expression
Alexander A. Green et al.
CELL (2014)
Paper-Based Synthetic Gene Networks
Keith Pardee et al.
CELL (2014)
Towards Personalized Medicine Mediated by in Vitro Virus-Based Interactome Approaches
Hiroyuki Ohashi et al.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2014)
Pharmacology, immunogenicity, and efficacy of a novel pegylated recombinant Erwinia chrysanthemi-derived L-asparaginase
Wei-Wen Chien et al.
INVESTIGATIONAL NEW DRUGS (2014)
Engineering toward a bacterial endoplasmic reticulum for the rapid expression of immunoglobulin proteins
Dan Groff et al.
MABS (2014)
Cell-free unnatural amino acid incorporation with alternative energy systems and linear expression templates
Prashanta Shrestha et al.
NEW BIOTECHNOLOGY (2014)
The road from next-generation sequencing to personalized medicine
Manuel L. Gonzalez-Garay
PERSONALIZED MEDICINE (2014)
Production and stabilization of the trimeric influenza hemagglutinin stem domain for potentially broadly protective influenza vaccines
Yuan Lu et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2014)
In vitro Fab display: a cell-free system for IgG discovery
Ryan L. Stafford et al.
PROTEIN ENGINEERING DESIGN & SELECTION (2014)
Linear DNA for Rapid Prototyping of Synthetic Biological Circuits in an Escherichia coli Based TX-TL Cell-Free System
Zachary Z. Sun et al.
ACS SYNTHETIC BIOLOGY (2014)
Cell-free Protein Synthesis from a Release Factor 1 Deficient Escherichia coli Activates Efficient and Multiple Site-specific Nonstandard Amino Acid Incorporation
Seok Hoon Hong et al.
ACS SYNTHETIC BIOLOGY (2014)
Gene Circuit Performance Characterization and Resource Usage in a Cell-Free Breadboard
Dan Siegal-Gaskins et al.
ACS SYNTHETIC BIOLOGY (2014)
Non-standard amino acid incorporation into proteins using Escherichia coli cell-free protein synthesis
Seok Hoon Hong et al.
FRONTIERS IN CHEMISTRY (2014)
Cell-free platforms for flexible expression and screening of enzymes
Christy Catherine et al.
BIOTECHNOLOGY ADVANCES (2013)
Enhanced protein stability through minimally invasive, direct, covalent, and site-specific immobilization
Mark T. Smith et al.
BIOTECHNOLOGY PROGRESS (2013)
Virus-like particles: the future of microbial factories and cell-free systems as platforms for vaccine development
William A. Rodriguez-Limas et al.
CURRENT OPINION IN BIOTECHNOLOGY (2013)
Translation Enhancer Improves the Ribosome Liberation from Translation Initiation
Shuntaro Takahashi et al.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2013)
In Vitro Reconstitution and Analysis of the 6-Deoxyerythronolide B Synthase
Brian Lowry et al.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2013)
Recent advances in the heterologous expression of microbial natural product biosynthetic pathways
Sarah E. Ongley et al.
NATURAL PRODUCT REPORTS (2013)
Cell-free co-production of an orthogonal transfer RNA activates efficient site-specific non-natural amino acid incorporation
Cem Albayrak et al.
NUCLEIC ACIDS RESEARCH (2013)
Validation of an entirely in vitro approach for rapid prototyping of DNA regulatory elements for synthetic biology
James Chappell et al.
NUCLEIC ACIDS RESEARCH (2013)
Reconstitution of eukaryotic translation initiation factor 3 by co-expression of the subunits in a human cell-derived in vitro protein synthesis system
Mamiko Masutani et al.
PROTEIN EXPRESSION AND PURIFICATION (2013)
Preparative Purification of Recombinant Proteins: Current Status and Future Trends
Mayank Saraswat et al.
BIOMED RESEARCH INTERNATIONAL (2013)
Nucleic acid amplification: Alternative methods of polymerase chain reaction
Md. Fakruddin et al.
JOURNAL OF PHARMACY AND BIOALLIED SCIENCES (2013)
Transforming Biochemical Engineering with Cell-Free Biology
James R. Swartz
AICHE JOURNAL (2012)
Cell-free protein synthesis: Applications come of age
Erik D. Carlson et al.
BIOTECHNOLOGY ADVANCES (2012)
Pegylated granulocyte colony-stimulating factor conveys long-term neuroprotection and improves functional outcome in a model of Parkinson's disease
Tobias Frank et al.
BRAIN (2012)
Experiment and mathematical modeling of gene expression dynamics in a cell-free system
Tobias Stoegbauer et al.
INTEGRATIVE BIOLOGY (2012)
Pilot Study of Granulocyte-Colony Stimulating Factor for Treatment of Alzheimer's Disease
Juan Sanchez-Ramos et al.
JOURNAL OF ALZHEIMERS DISEASE (2012)
Multiple Post-translational Modifications Affect Heterologous Protein Synthesis
Alexander A. Tokmakov et al.
JOURNAL OF BIOLOGICAL CHEMISTRY (2012)
Glycosyltransferase and its application to glycodiversification of natural products
Sung Hee Choi et al.
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY (2012)
Aglycosylated antibodies and antibody fragments produced in a scalable in vitro transcription-translation system
Gang Yin et al.
MABS (2012)
Affinity-purification coupled to mass spectrometry: Basic principles and strategies
Wade H. Dunham et al.
PROTEOMICS (2012)
Yeast two-hybrid methods and their applications in drug discovery
Amel Hamdi et al.
TRENDS IN PHARMACOLOGICAL SCIENCES (2012)
Streamlined extract preparation for Escherichia coli-based cell-free protein synthesis by sonication or bead vortex mixing
Prashanta Shrestha et al.
BIOTECHNIQUES (2012)
Microscale to Manufacturing Scale-up of Cell-Free Cytokine Production-A New Approach for Shortening Protein Production Development Timelines
James F. Zawada et al.
BIOTECHNOLOGY AND BIOENGINEERING (2011)
L-Asparaginase Treatment in Acute Lymphoblastic Leukemia
Rob Pieters et al.
CANCER (2011)
The Agrobacterium tumefaciens Transcription Factor BlcR Is Regulated via Oligomerization
Yi Pan et al.
JOURNAL OF BIOLOGICAL CHEMISTRY (2011)
Efficient disulfide bond formation in virus-like particles
Bradley C. Bundy et al.
JOURNAL OF BIOTECHNOLOGY (2011)
Label-Free, Single Protein Detection on a Near-Infrared Fluorescent Single-Walled Carbon Nanotube/Protein Microarray Fabricated by Cell-Free Synthesis
Jin-Ho Ahn et al.
NANO LETTERS (2011)
Coarse-Grained Dynamics of Protein Synthesis in a Cell-Free System
Eyal Karzbrun et al.
PHYSICAL REVIEW LETTERS (2011)
Site-Specific Incorporation of p-Propargyloxyphenylalanine in a Cell-Free Environment for Direct Protein-Protein Click Conjugation
Bradley C. Bundy et al.
BIOCONJUGATE CHEMISTRY (2010)
Cell-free production of functional antibody fragments
In-Seok Oh et al.
BIOPROCESS AND BIOSYSTEMS ENGINEERING (2010)
A Highly Controllable Reconstituted Cell-Free System -a Breakthrough in Protein Synthesis Research
Hiroyuki Ohashi et al.
CURRENT PHARMACEUTICAL BIOTECHNOLOGY (2010)
Simple Screening Method for Autoantigen Proteins Using the N-Terminal Biotinylated Protein Library Produced by Wheat Cell-Free Synthesis
Kazuhiro Matsuoka et al.
JOURNAL OF PROTEOME RESEARCH (2010)
Protein synthesis in a device with nanoporous membranes and microchannels
Qian Mei et al.
LAB ON A CHIP (2010)
Determinants of Initiation Codon Selection during Translation in Mammalian Cells
Daiki Matsuda et al.
PLOS ONE (2010)
Cell-Free Synthesis of Functional Aquaporin Z in Synthetic Liposomes
Norman T. Hovijitra et al.
BIOTECHNOLOGY AND BIOENGINEERING (2009)
The MerE protein encoded by transposon Tn21 is a broad mercury transporter in Escherichia coli
Masako Kiyono et al.
FEBS LETTERS (2009)
The malaria candidate vaccine liver stage antigen-3 is highly conserved in Plasmodium falciparum isolates from diverse geographical areas
Eric Prieur et al.
MALARIA JOURNAL (2009)
Drivers of biodiagnostic development
David A. Giljohann et al.
NATURE (2009)
Cell-free protein synthesis system from Escherichia coli cells cultured at decreased temperatures improves productivity by decreasing DNA template degradation
Eiko Seki et al.
ANALYTICAL BIOCHEMISTRY (2008)
Evaluation of cell-free protein synthesis using PDMS-based microreactor Arrays
Takatoki Yamamoto et al.
ANALYTICAL SCIENCES (2008)
Escherichia coli-based cell-free synthesis of virus-like particles
Bradley C. Bundy et al.
BIOTECHNOLOGY AND BIOENGINEERING (2008)
At in vitro strategy for the rapid expression of recombinant proteins at low temperature
Ju-Young Byun et al.
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING (2008)
Granulocyte-colony stimulating factor improves outcome in a mouse model of amyotrophic lateral sclerosis
Claudia Pitzer et al.
BRAIN (2008)
An optimized yeast cell-free system: Sufficient for translation of human papillomavirus 58 L1 mRNA and assembly of virus-like particles
Xiao Wang et al.
JOURNAL OF BIOSCIENCE AND BIOENGINEERING (2008)
Protein production and purification
Susanne Graslund et al.
NATURE METHODS (2008)
Development of cell-free protein synthesis platforms for disulfide bonded proteins
Aaron R. Goerke et al.
BIOTECHNOLOGY AND BIOENGINEERING (2008)
Cell-free protein synthesis in microfluidic array devices
Qian Mei et al.
BIOTECHNOLOGY PROGRESS (2007)
Cell-free synthesis of proteins that require disulfide bonds using glucose as an energy source
Kurtis G. Knapp et al.
BIOTECHNOLOGY AND BIOENGINEERING (2007)
Chaperone-based procedure to increase yields of soluble recombinant proteins produced in E-coli
Ario de Marco et al.
BMC BIOTECHNOLOGY (2007)
Reconstitution of the biochemical activities of the AttJ repressor and the AttK, AttL, and AttM catabolic enzymes of Agrobacterium tumefaciens
Yunrong Chai et al.
JOURNAL OF BACTERIOLOGY (2007)
Hematopoietic growth factors pass through the blood-brain barrier in intact rats
Li-Ru Zhao et al.
EXPERIMENTAL NEUROLOGY (2007)
Domain-swapped dimerization of the HIV-1 capsid C-terminal domain
Dmitri Ivanov et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2007)
Fluorescent labeling of cell-free synthesized proteins by incorporation of fluorophore-conjugated nonnatural amino acids
Sang-Hyeon Kang et al.
ANALYTICAL BIOCHEMISTRY (2007)
Ribosomal tethering and clustering as mechanisms for translation initiation
Stephen A. Chappell et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2006)
Providing an oxidizing environment for the cell-free expression of disulfide-containing proteins by exhausting the reducing activity of Escherichia coli S30 extract
In-Seok Oh et al.
BIOTECHNOLOGY PROGRESS (2006)
DNA detection using recombination proteins
Olaf Piepenburg et al.
PLOS BIOLOGY (2006)
Adverse effects of the model environmental estrogen diethylstilbestrol are transmitted to subsequent generations
Retha R. Newbold et al.
ENDOCRINOLOGY (2006)
Enhancing the efficiency of cell-free protein synthesis through the polymerase-chain-reaction-based addition of a translation enhancer sequence and the in situ removal of the extra amino acid residues
JM Son et al.
ANALYTICAL BIOCHEMISTRY (2006)
Testicular dysgenesis syndrome: possible role of endocrine disrupters
K Bay et al.
BEST PRACTICE & RESEARCH CLINICAL ENDOCRINOLOGY & METABOLISM (2006)
Cell-free translation systems for protein engineering
Yoshihiro Shimizu et al.
FEBS JOURNAL (2006)
Cell-free synthesis of recombinant proteins from PCR-amplified genes at a comparable productivity to that of plasmid-based reactions
JH Ahn et al.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2005)
Engineering thermal stability in RNA phage capsids via disulphide bonds
AE Ashcroft et al.
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY (2005)
Cell-free production of aggregation-prone proteins in soluble and active forms
SH Kang et al.
BIOTECHNOLOGY PROGRESS (2005)
Quantitative polysome analysis identifies limitations in bacterial cell-free protein synthesis
KA Underwood et al.
BIOTECHNOLOGY AND BIOENGINEERING (2005)
Energizing cell-free protein synthesis with glucose metabolism
KA Calhoun et al.
BIOTECHNOLOGY AND BIOENGINEERING (2005)
Soluble expression of recombinant proteins in the cytoplasm of Escherichia coli -: art. no. 1
HP Sorensen et al.
MICROBIAL CELL FACTORIES (2005)
Effect of N-terminal and Met23 mutations on the structure and dynamics of onconase
VY Gorbatyuk et al.
JOURNAL OF BIOLOGICAL CHEMISTRY (2004)
Efficient production of a bioactive, multiple disulfide-bonded protein using modified extracts of Escherichia coli
DM Kim et al.
BIOTECHNOLOGY AND BIOENGINEERING (2004)
Substrate replenishment extends protein synthesis with an in vitro translation system designed to mimic the cytoplasm
MC Jewett et al.
BIOTECHNOLOGY AND BIOENGINEERING (2004)
A cell-free biosensor for the detection of transcriptional inducers using firefly luciferase as a reporter
T Pellinen et al.
ANALYTICAL BIOCHEMISTRY (2004)
Are all EDC effects mediated via steroid hormone receptors?
JS Fisher
TOXICOLOGY (2004)
Recombinant protein folding and misfolding in Escherichia coli
F Baneyx et al.
NATURE BIOTECHNOLOGY (2004)
Why is trehalose an exceptional protein stabilizer? An analysis of the thermal stability of proteins in the presence of the compatible osmolyte trehalose
JK Kaushik et al.
JOURNAL OF BIOLOGICAL CHEMISTRY (2003)
Hepatitis C virus-like particle morphogenesis
E Blanchard et al.
JOURNAL OF VIROLOGY (2002)
Disulfide bonds stabilize JC virus capsid-like structure by protecting calcium ions from chelation
PL Chen et al.
FEBS LETTERS (2001)
Oxalate improves protein synthesis by enhancing ATP supply in a cell-free system derived from Escherichia coli
DM Kim et al.
BIOTECHNOLOGY LETTERS (2000)
Share Paper
