Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 583, Issue -, Pages 128-134Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2021.10.064
Keywords
Clusterin; Preeclampsia; Epithelial-mesenchymal transition; Invasion
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Funding
- National Natural Science Fund of China [81871716]
- Science and Technology project Fund of Guangzhou [20191A011035]
- Natural Science Fund of Guangdong Province [2018A0303130314]
- Guangzhou Institute of Pediatrics/Guangzhou Women and Children's Medical Center [IP-2019-009]
- Guangzhou Women and Children's Medical Center [CWCMC2020-6-017]
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The study found that the expression of CLU is increased before the onset of PE and is positively correlated with the severity of the condition. CLU can inhibit the epithelial-mesenchymal transition of trophoblast cells, thereby reducing cell migration and invasion.
Preeclampsia (PE) threatens the safety of mothers and fetuses, and its pathogenesis is still unclear. Our previous study has found the relationship between PE and serum Clusterin (CLU). This study aimed to investigate the role of CLU on PE. Firstly, levels of CLU in serum and placental tissue from PE patients and healthy pregnancies were compared. Then, RNA sequencing, cell counting kit-8, matrigel invasion, cell apoptosis, and angiogenesis assay were performed to evaluate the role of CLU on primary isolation trophoblast cells. We found the expression of CLU was increased before the clinical syndrome occurred, whereas its level was positively related to the severity of PE. CLU significantly inhibited the expression of matrix metalloproteinase-9 and Vimentin and enhanced E-cadherin to inhibit epithelial-mesenchymal transition of trophoblast cells, further reducing its migration and invasion. Our results suggested that CLU may play a role in regulating trophoblast invasion and migration during placental development, which may be one of the risk factors for PE. (c) 2021 Elsevier Inc. All rights reserved.
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