Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 589, Issue -, Pages 9-15Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2021.11.092
Keywords
DNA second Structure; i-motif; Binding compounds; Transcription factor; BmILF
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Funding
- Chinese National Natural Science Foundation [31672494, 31720103916, 31930102]
- Guangdong Basic and Applied Basic Research Foundation [2019A1515110784]
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In this study, a large-scale screening of binding compounds targeting the i-motif structure of BmPOUM2 was conducted using surface plasmon resonance imaging (SPRi), resulting in the identification of several compounds with binding affinity to the i-motif structure. These compounds have potential applications in gene transcription regulation and functional analysis of i-motif structure.
DNA secondary structure i-motif involves in gene transcription and considered as a novel target for cancer gene therapy. I-motif-binding compounds can either stabilize or destroy the structure, resulting in change in target gene transcription. In this study, a large-scale screening of binding compounds was conducted using the i-motif structure of BmPOUM2, a transcription factor in silkworm, Bombyx mori. Surface plasmon resonance imaging (SPRi) high-throughput binding screening of 3642 compounds found 60 compounds with an binding affinity K-d of 10(-7)-10(-6) M. SPRi and circular dichroism (CD) double screening demonstrated that the BmPOUM2 i-motif structure bound the compounds IF1, IF3, IF4, IF6 and IF7 with K-d of 10(-7) M, and the compounds IF2 and tetrakis (4-N-methylpyridyl) porphine (TMPyP4) with a K-d of 10(-8) M. Interestingly, IF2, IF3, IF4, IF6 and IF7 promoted the binding of the i-motif-binding protein BmILF with the i-motif structure, whereas TMPyP4 inhibited the binding. This study provided a list of compounds that have potential applications in functional analysis of i-motif structure and in pesticide and drug development through gene transcription regulation by i-motif structure. (C) 2021 The Authors. Published by Elsevier Inc.
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