The reductive carboxylation activity of heterotetrameric pyruvate synthases from hyperthermophilic archaea

Pyruvate synthase (pyruvate:ferredoxin oxidoreductase, PFOR) catalyzes the interconversion of acetylCoA and pyruvate, but the reductive carboxylation activities of heterotetrameric PFORs remain largely unknown. In this study, we cloned, expressed, and purified selected six heterotetrameric PFORs from hyperthermophilic archaea. The reductive carboxylation activities of these heterotetrameric PFORs were measured at 70 degrees C and the ratio of reductive carboxylation activity to oxidative decarboxylation activity (red/ox ratio) were calculated. Four out of six showed reductive decarboxylation activities. Among them, the PFORpfm from Pyrolobus fumarii showed the highest reductive carboxylation activities and the highest red/ox ratio, which were 54.32 mU/mg and 0.51, respectively. The divergence of the reductive carboxylation activities and the red/ox ratios of heterotetrameric PFORs in hyperthermophilic archaea indicate the diversity of the functions of PFOR over long-term evolution. This can help us better understand the autotrophic CO2 fixation process in thermal vent, or in other CO2-rich high temperature habitat. (C) 2021 Elsevier Inc. All rights reserved.

Automated summary

This study investigated the reductive carboxylation activities of six heterotetrameric PFORs from hyperthermophilic archaea, revealing diversity in their functions. Among them, PFORpfm from Pyrolobus fumarii showed the highest reductive carboxylation activities and red/ox ratio, indicating the potential significance of PFOR in autotrophic CO2 fixation in high temperature environments.