Journal
ARCHIVES OF TOXICOLOGY
Volume 96, Issue 2, Pages 525-533Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00204-021-03209-7
Keywords
Gelsenicine; UPLC-Q-TOF-MS; Metabolic profiling; Toxicokinetics; Metabolism
Categories
Funding
- National Natural Science Foundation of China [81773691, 81973696]
- Wenzhou Science and Technology Major Project, China [ZS2017018]
Ask authors/readers for more resources
This study investigated the metabolic profile and toxicokinetics of gelsenicine in rats using UPLC and mass spectrometry. The results revealed that gelsenicine is mainly metabolized in the liver and excreted through urine and bile. This study provides important information for the risk assessment and forensic identification of gelsenicine.
Gelsenicine, mainly isolated from Gelsemium elegans Benth., is one of the most toxic alkaloids. The lack of information on gelsenicine leads to inaccurate risk and poisoning evaluation. In this study, the metabolic profiling and toxicokinetics of gelsenicine was studied by ultra-high performance liquid chromatography (UPLC) with quadrupole time-of-flight (Q-ToF) and tandem mass spectrometry in rats after intraperitoneal (i.p., 40 mu g/kg) and intragastric (i.g., 60 mu g/kg) administration. After i.p. administration, the area under the curve (AUC), the apparent volume of distribution (V), and the total body clearance (CL/F) of gelsenicine in plasma were 3.79 mu g/L h, 38.47 L/kg, and 11.87 mL/h kg, respectively. After i.g. administration, the corresponding values were slightly increased (5.49 mu g/L h; 53.10 mL/kg, and 12.66 mL/h kg). The toxicokinetic results indicated that the hepatic first-pass effect was predominant after i.p. administration. The UPLC-Q-ToF-MS data revealed nine metabolites in plasma, urine, and bile which were largely obtained by demethylation, hydroxylation, acetylation and glycine conjugation. Metabolites were mainly excreted through urine and bile, most of which in urine was basically eliminated in 24 h. Molecular docking and liver microsome experiments further showed that gelsenicine was metabolized by cytochrome P450 3A4 and 3A5. Summarizing, the present study provides metabolic and toxicokinetic information on gelsenicine which in turn may help in future risk assessment and forensic identification after poisonings.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available