Rhabdonatronobacter sediminivivens gen. nov., sp. nov. isolated from the sediment of Hutong Qagan Soda Lake

A novel Gram-stain-negative bacterium, designated as IM2376(T), was isolated from the sediment of Hutong Qagan Lake in the Ordos, Inner Mongolia Autonomous Region of China. Phylogenetic analysis based on 16S rRNA gene sequence revealed that the strain IM2376(T) had the highest similarity with Roseinatronobacter thiooxidans DSM 13087(T) (96.2%) and Rhodobaca bogoriensis LBB1(T) (96.2%) of the family Rhodobacteraceae. Genomic relatedness analyses showed that strain IM2376(T) was clearly distinguished from other species in the family Rhodobacteraceae, with average nucleotide identities, average amino acid identities, and in silico DNA-DNA hybridization values not more than 74.1, 68.5, and 20.2%, respectively. The fatty acids were mainly composed of C-18:1 omega 7c (64.9%), iso-C-16:0 (16.3%), and C-16: 1 omega 7c/C-16:1 omega 6c (6.0%). The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylcholine. The predominant ubiquinone was Q-10 (94.9%). The genomic DNA G + C content was 66 mol%. Based on all these results, strain IM2376(T) was considered a novel species of a new genus in the family Rhodobacteraceae, for which the name Rhabdonatronobacter sediminivivens gen. nov., sp. nov. is proposed. The type strain of Rhabdonatronobacter sediminivivens is IM2376(T) (= CGMCC 1.17852(T) = KCTC 92134(T)).

Automated summary

A novel Gram-stain-negative bacterium, named Rhabdonatronobacter sediminivivens, was isolated from the sediment of Hutong Qagan Lake in Inner Mongolia Autonomous Region, China. It showed distinct phylogenetic differences from other species in the family Rhodobacteraceae. Based on its fatty acid composition, polar lipid profiles, and genomic characteristics, it can be classified as a new genus and species.