Paenibacillus arenosi sp. nov., a siderophore-producing bacterium isolated from coastal sediment

In this study, strain CAU 1523(T), a novel Gram-positive-positive bacterium isolated from marine sediment collected from the coast of Busan, Republic of Korea, was characterized using a polyphasic taxonomic approach. This strain showed growth at a temperature range of 20-37 degrees C (optimum, 30 degrees C), a pH range of 6.5-9.5 (optimum, 7.5), and in the presence of 0-3% (w/v) NaCl (optimum, 1%). Phylogenetic analysis based on 16S rRNA gene sequencing and 92 concatenated core genes indicated that CAU 1523(T) belonged to the genus Paenibacillus, sharing the highest sequence similarity with P. assamensis JCM 13186(T) (98.0%). CAU 1523(T) was differentiated from other Paenibacillus species by average nucleotide identity, average amino acid identity, and digital DNA-DNA hybridization values, using cut-off values of 95-96%, 90%, and 70%, respectively, for closely related strains. The genome of CAU 1523(T) possessed various biosynthetic gene clusters, one of which encoded a putative siderophore-interacting protein. Siderophore production by the isolate was confirmed using the qualitative chrome azurol sulfonate (CAS) agar assay. Based on its phylogenetic and physiological characteristics, strain CAU 1523(T) represents a novel, siderophore-producing species within the genus Paenibacillus, for which the name Paenibacillus arenosi sp. nov. is proposed, with the type strain CAU 1523(T) (= KCTC 43108(T) = MCCC 1K04063(T)).

Automated summary

In this study, a novel Gram-positive bacterium, strain CAU 1523(T), isolated from marine sediment collected from the coast of Busan, Republic of Korea, was characterized using a polyphasic taxonomic approach. Phylogenetic and physiological analysis revealed that CAU 1523(T) represents a new siderophore-producing species within the genus Paenibacillus, and it was named Paenibacillus arenosi sp. nov. The strain exhibited specific growth conditions and possessed various biosynthetic gene clusters.