Mitochondrial genomes of 10 Mantidae species and their phylogenetic implications

This article aims to present a phylogenetic evaluation of Mantidae based on a mitochondrial genome (mitogenome) data set. The mitogenome of 10 Mantidae species were sequenced using next-generation sequencing. The length of nine the complete mitogenomes ranged from 15,371 bp in Tenodera aridifolia to 16,063 bp in Hierodula longa. Mantidae mitogenomes have 37 genes and control region with two exceptions: five trnR copies in Statilia maculata, and H. zhangi was incomplete missing trnI, trnQ, trnM and a portion of the control region. There was a large noncoding region (LNC) between trnM and nad2 in H. chinensis, H. longa, H. maculata and Titanodula sp. Most of protein-coding genes (PCGs) used the typical start ATN codon and TAA/TAG stop codons. All tRNAs fold into the typical clover-leaf secondary structure except trnS1 which lacks a dihydrouracil (DHU) arm. Nucleotide diversity and Ka/Ks analysis of 13 PCGs showed that atp8 had the highest variability and fastest evolutionary rate. Phylogenetic relationships among 42 Mantidae species were reconstructed using the 13 PCGs and two rRNA genes using Bayesian Inference (BI) and Maximum Likelihood (ML) methods. Of the seven mantid subfamilies included in this analysis, only four had multiple exemplars, and of those only Mantinae and Vatinae formed monophyletic groups in BI and ML trees. Consistent with previous studies, the monophyly of the Hierudulinae and Tenoderinae were not been supported. The present results imply that it is necessary to combine nuclear molecular markers and external characteristic to understand the phylogenetic relationships within Mantidae.

Automated summary

This article presents a phylogenetic evaluation of Mantidae based on a mitochondrial genome data set. The mitogenome of 10 Mantidae species were sequenced and analyzed. The results revealed specific genes and control regions in Mantidae mitogenomes. Nucleotide diversity and Ka/Ks analysis showed variation and evolutionary rate differences in certain genes. The reconstruction of phylogenetic relationships highlighted the monophyletic groups formed by Mantinae and Vatinae subfamilies, while the monophyly of other subfamilies was not supported.