Journal
AQUACULTURE RESEARCH
Volume 53, Issue 5, Pages 1873-1889Publisher
WILEY
DOI: 10.1111/are.15715
Keywords
aquaculture; fermentation; probiotic; Rhynchocypris lagowskii
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Funding
- Jilin Provincial Science, Technology Department [20180201017NY]
- Jilin Province Education Department Science and Technology Project [JJKH20210370KJ]
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The bacterial strain LSG2-8 isolated from the intestine of Rhynchocypris lagowskii showed potential for feed fermentation, with good characteristics in terms of safety, adhesion, and fermentation conditions.
In this study, 30 candidate bacterial strains were isolated from the intestine of Rhynchocypris lagowskii. LSG2-8 was screened out through enzyme production test, antibiotic susceptibility test and anti-pathogens ability. The LSG2-8 was identified as Bacillus amyloliquefaciens. Bio-safety assay confirmed that the LSG2-8 was not pathogenic to the host fish and performed better in the tolerance test. The results of the adherence ability showed that the adhesion rate of LSG2-8 was 36% in vitro intestinal mucosal adhesion model. Analysis of their surface-adhesive proteins revealed that LSG2-8 bacterial cell surface lectin mainly exhibited glycoprotein properties. The results of the interaction test on temperature, initial pH and inoculation amount showed that the optimum fermentation conditions for the LSG2-8 were 30 degrees C, 6.0 and 4%, respectively. In summary, the B. amyloliquefaciens LSG2-8 from the intestine of R. lagowskii is a promising candidate strain for feed fermentation.
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