Journal
AQUACULTURE
Volume 549, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.aquaculture.2021.737780
Keywords
Shrimp; Arachidonic acid; Microcystin-LR; Intestinal microbial; Immune gene
Categories
Funding
- National Natural Science Foundation of China [31902343]
- Guangdong Provincial Special Fund for Modern Agriculture Industry Technology Innovation Teams [2019KJ141]
- Young Scientific Talents Lifting Planning Project of Guangzhou Science and Technology Association [X20210201039]
- Central Public-interest Scientific Institution Basal Research Fund, South China Sea Fisheries Research Institute, CAFS [2021SD19]
- Guangdong Natural Science Foundation [2021A1515012084]
- Guangzhou Science and Technology Plan Project [202102080246]
- Agricultural Research Outstanding Talents Training Program [13210308]
- Central Public-interest Scientific Institution Basal Research Fund, CAFS [2021XT0603]
- National Key Research and Development Program of China [2019YFD0900500]
- China-ASEAN Maritime Cooperation Fund
- China-ASEAN Modern Marine Fishery Technical Cooperation, Industrialization Development and Demonstration
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This study examined the effects of dietary AA on the growth, immunity, and intestinal microbiota of Litopenaeus vannamei under MC-LR stress. The results showed that dietary AA improved the growth and feed utilization of the shrimp and modulated immune response and intestinal microbial composition.
The harmful effects of microcystin-LR (MC-LR) stress are unavoidable in shrimp culture. Arachidonic acid (AA) is a fatty acid that regulates immune responses in aquatic animals. In this study, we investigated the effects of dietary AA on growth, immunity and intestinal microbiota of Litopenaeus vannamei under MC-LR stress. The shrimp were fed a control diet (non-supplemented with AA) or a AA diet (supplemented with 8.0 g/kg AA) for 56 days, followed by an acute MC-LR stress for 72 h. The results showed that dietary AA improved the growth and feed utilization of the shrimp. Microcystin-LR exposure increased the mRNA expressions of reactive oxygen species modulator 1 (ROMO1), glutathione peroxidase (GPx), caspase-3 (Casp-3), NADPH-cytochrome P450 reductase (NCPR) and sulfotransferase (SULT), but decreased that of catalase (CAT) and cytochrome C (Cytc). Dietary AA supplementation reversed the expressions of ROMO1, CAT, GPx and NCPR to the control level, but still maintained the higher levels of Cytc, Casp-3 and SULT than the control and MC-LR stress groups. Dietary AA could not effectively reverse the changes of intestinal microbial diversity, but it could improve intestinal microbial composition variation induced by MC-LR stress. Specially, dietary AA increased the relative abundances of beneficial bacteria Bacteroides and Lactobacillus, and reversed the changes of pathogenic bacteria Vibrio and Photobacterium induced by MC-LR stress to the control level. The changes of intestinal bacteria were correlated with immune gene expression. These results revealed that dietary AA had a positive effect on L. vannamei resistance to MC-LR stress by modulating immune response and intestinal microbial composition.
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