Inhibition of biofilm formation induced by functional graphenic materials impregnated in Nile tilapia (Oreochromis niloticus) skin

Wound dressings based on natural materials, such as fish skin, represent an important strategy for the treatment of burns. Despite their utility, contamination of these natural materials with bacteria (planktonic and biofilm forms) introduces significant risks to patients under treatment. This disadvantage can be overcome by modifying the material's surface to prevent bacterial deposition through chemical or physical interactions. In this work, functional graphenic materials (FGM) with tunable surface charges were incorporated into tilapia (Oreochromis niloticus) fish skin as a part of a strategy to control the biofilm adhesion on surfaces. The antibiofilm activity was evaluated against S. aureus and K. pneumoniae due to the biofilm-forming properties of these bacterial strains. FGM-modified tilapia skin samples possess a strong capacity to reduce biofilm formation on the tilapia fish skin with a higher antibiofilm activity against Gram-positive bacteria, compared to Gram-negative bacteria. Nega-tively charged FGMs were more effective than positively charged FGMs in preventing biofilm formation on the impregnated tilapia skin xenografts: negatively charged Claisen graphene achieved an 88.8% reduction in bio-film formation on the tilapia skin. Overall, this study demonstrates the utility of FGM-impregnated tilapia skins as a treatment for burn wounds due to their ability to modulate bacterial adhesion.

Automated summary

This study demonstrates the effectiveness of functional graphenic materials (FGM) incorporated into tilapia fish skin in reducing biofilm formation, especially with higher antibiofilm activity against Gram-positive bacteria. Negatively charged FGMs were more effective in preventing biofilm formation, indicating potential for FGM-impregnated tilapia skins as a treatment for burn wounds.