High-intensity interval resistance training (HIIRT) improves liver gluconeogenesis from lactate in Swiss mice

High-intensity physical exercise favors anaerobic glycolysis and increases lactatemia. Lactate is converted back to glucose in the liver, so that the lactate threshold, an indicator of physical performance, must be related to the gluconeo-genic capacity of the liver. This research assessed the effect of a high-intensity interval resistance training (HIIRT) on liver gluconeogenesis from lactate. Swiss mice were trained (groups T) on vertical ladder with overload of 90% of their maximal load. Control animals remained untrained (groups C0 and C8). In situ liver perfusion with lactate and adrenaline was per -formed in rested mice after 6 hours of food deprivation. There were larger outputs of glucose (T6, 71.90%; T8, 54.53%) and pyruvate (T8, 129.28%) (representative values for 4 mM lactate) in the groups trained for 6 or 8 weeks (T6 and T8), and of glucose in the presence of adrenaline in group T8 (280%). The content of PEPCK, an important regulatory enzyme of the gluconeogenic pathway, was 69.13% higher in group T8 than in the age-matched untrained animals (C8). HIIRT augmented liver gluconeogenesis from lactate and this might improve the lactate threshold. Novelty: center dot The liver metabolizes lactate from muscle into glucose. center dot Physical training may enhance the gluconeogenic capacity of the liver. center dot As lactate clearance by the liver improves, lactate threshold is displaced to higher exercise intensities.

Automated summary

This study evaluated the effect of high-intensity interval resistance training on liver gluconeogenesis from lactate. The results showed that mice trained for 6 or 8 weeks had higher glucose and pyruvate production from lactate compared to untrained mice. The content of the key regulatory enzyme PEPCK was also increased in the trained group. These findings suggest that high-intensity training can improve the lactate threshold by enhancing liver gluconeogenesis from lactate.