Journal
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
Volume 106, Issue 3, Pages 1045-1056Publisher
SPRINGER
DOI: 10.1007/s00253-022-11780-x
Keywords
Palm kernel cake; Enzymolysis; Mannose; Manno-oligosaccharides; Solid-state fermentation; Yeast culture
Categories
Funding
- Open Funds of the State Key Laboratory of Agricultural Microbiology [AMLKF201905]
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This study proposes a novel approach to produce feed additives, including MMOM and YC, with high value by utilizing PKC through enzymolysis and solid-state fermentation.
Palm kernel cake (PKC) is an agricultural waste derived from palm kernel oil manufacturing, and its production is increasing year by year. It is very urgent to process this agricultural waste in an environmentally friendly way. Here, PKC was used to produce mannose and manno-oligosaccharides mixture (MMOM) and yeast culture (YC) through enzymolysis and solid-state fermentation (SSF). In enzymolysis, five factors were optimized separately and a response surface methodology analysis was performed. Then, enzymolysis of PKC was carried out at the optimal condition, and the extraction efficiency of mannose and manno-oligosaccharides reached 68.90% with mannose concentration achieving 60.27 g/L. After enzymolysis, the enzymatic hydrolysate was dried by spray drying, and the contents of MMOM reached 42.9%. In SSF, the enzymolysis residues were utilized with inoculating Saccharomyces cerevisiae for yielding YC. After optimization, the cells number of S. cerevisiae reached 2.08 x 10(9) cells/g and the crude protein content was increased to 27.31%. Therefore, a novel approach to produce feed additives, including MMOM and YC, with high value by comprehensive utilization of PKC was proposed, which has good application prospects in the breeding industry.
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