Journal
ANTICANCER RESEARCH
Volume 41, Issue 11, Pages 5481-5488Publisher
INT INST ANTICANCER RESEARCH
DOI: 10.21873/anticanres.15361
Keywords
ALDH; cancer stem cells (CSCs); CSC markers; A549; HepG2
Categories
Funding
- Bodossaki Foundation PhD scholarship
- Hellenic Foundation for Research and Innovation (HFRI) [523]
- OPENSCREEN-GR: An Open-Access Research Infrastructure of Target-Based Screening Technologies and Chemical Biology for Human and Animal Health, Agriculture and Environment [MIS 5002691]
- Operational Programme Competitiveness, Entrepreneurship and Innovation
- European Union (European Regional Development Fund)
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Specific ALDH isoforms are important mediators for the acquisition of a CSC phenotype in non-small cell lung and hepatocellular carcinomas, suggesting their potential as promising targets for therapeutic approaches.
Background/Aim: Aldehyde dehydrogenases (ALDHs) are considered as markers for normal and cancer stem cells (CSC) and are involved in cell metabolism, proliferation, differentiation, stemness, and retinoic acid (RA) biosynthesis. The aim of the present study was to identify the ALDH isoforms that are associated with the CSC phenotype in non-small cell lung and hepatocellular carcinomas. Materials and Methods: We utilized lung (A549) and hepatocellular (HepG2) cancer cells and generated tumor spheres to isolate the CSC sub-population. Results: The CSC enrichment was confirmed by the up-regulation of various CSC-related genes. Comparative qPCR analysis indicated the up-regulation of several ALDH isoforms in A549 and HepG2 spheres. Interestingly, cyclin D1 and Akt, down-stream targets of the RA signaling pathway, were also shown to be significantly up-regulated in both sphere populations. Conclusion: Specific ALDH isoforms appear to be important mediators for the acquisition of an CSC phenotype and thus, are potential promising targets for CSC-based therapeutic approaches in lung and hepatocellular carcinomas.
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