Journal
ANNALS OF MEDICINE
Volume 54, Issue 1, Pages 51-62Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/07853890.2021.2016942
Keywords
Fusobacterium nucleatum; oesophageal squamous cell carcinomas; CD8(+) T lymphocytes; KIR2DL1; prognosis
Categories
Funding
- Major Projects of Science and Technology Department in Anyang City [ZDKJJS2020006]
- Henan Provincial Key Science and Technology Project [212102310670]
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Fn infection is significantly correlated with high KIR2DL1 expression on CD8(+) T cells, leading to poor tumor differentiation, advanced clinical stage, and short survival time in ESCC patients. Fn infection and CD8(+)KIR2DL1 positive group are independent risk factors affecting the prognosis of ESCC patients.
Background To analyze the correlation between the inducing effect of Fusobacterium nucleatum (Fn) on the surface expression of the inhibitory receptor KIR2DL1 on CD8(+) T cells in oesophageal squamous cell carcinoma (ESCC) and the clinicopathological features and survival prognosis and to explore its clinical significance. Methods The inducing effect of Fn on CD8(+) T cell surface inhibitory receptor KIR2DL1 expression was analyzed in a coculture system of human CD8(+) T cells and ESCC cells infected with Fn. Fn infection and the expression of KIR2DL1 on CD8(+) T cells were detected by RNAscope and immunohistochemistry in ESCC tissues, and the correlations between the inducing effect of Fn on KIR2DL1 expression on CD8(+) T cells and clinicopathological features were analyzed. COX regression was used to analyze the influence of each factor on the prognosis of ESCC. Survival curves were plotted by the Kaplan-Meier method, and the effect of KIR2DL1 induction on survival time was analyzed by the log-rank test. Results In the coculture system, KIR2DL1 expression on the surface of CD8(+) T cells increased with increasing Fn infection time. In ESCC tissues, Fn infection was significantly correlated with high KIR2DL1 expression on CD8(+) T cells. The Fn + CD8(+)KIR2DL1 positive patients were predominantly males who were smokers and alcohol drinkers. Moreover, patients with Fn infection were characterized by poor tumour differentiation, advanced clinical stage, and a short survival time. Meanwhile, Fn + CD8(+)KIR2DL1 positive group was independent risk factor affecting the prognosis of ESCC patients. Conclusions Long-term drinking and smoking lead to an extremely unhealthy oral environment in which Fn infection and colonization are more likely to occur, thus inducing high expression of KIR2DL1 on the surface of CD8(+) T cells, which can weaken the antitumour immune response and promote the malignant progression of ESCC.
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