Correlations between leaf economics, mechanical resistance and drought tolerance across 41 cycad species

Background and Aims We conducted a comprehensive analysis of the functional traits of leaves (leaflets) of cycads. The aim of this study was to clarify the functional divergence between the earlier origin Cycadaceae and the later differentiated Zamiaceae, and the differences in trait associations between cycads and angiosperms. Methods We selected 20 Cycadaceae species and 21 Zamiaceae species from the same cycad garden in South China, and measured their leaf structure, economic traits, mechanical resistance (F-p) and leaf water potential at the turgor loss point (pi(tlp)). In addition, we compiled a dataset of geographical distribution along with climatic variables for these cycad species, and some leaf traits of tropical-sub-tropical angiosperm woody species from the literature for comparison. Key Results The results showed significantly contrasting leaf trait syndromes between the two families, with Zamiaceae species exhibiting thicker leaves, higher carbon investments and greater F-p than Cycadaceae species. Leaf thickness (LT) and pi(tlp) were correlated with mean climatic variables in their native distribution ranges, indicating their evolutionary adaptation to environmental conditions. Compared with the leaves of angiosperms, the cycad leaves were thicker and tougher, and more tolerant to desiccation. Greater F-p was associated with a higher structural investment in both angiosperms and cycads; however, cycads showed lower F-p at a given leaf mass per area or LT than angiosperms. Enhancement of F-p led to more negative pi(tlp) in angiosperms, but the opposite trend was observed in cycads. Conclusions Our results reveal that variations in leaf traits of cycads are mainly influenced by taxonomy and the environment of their native range. We also demonstrate similar leaf functional associations in terms of economics, but different relationships with regard to mechanics and drought tolerance between cycads and angiosperms. This study expands our understanding of the ecological strategies and likely responses of cycads to future climate change.

Automated summary

This study conducted a comprehensive analysis of the functional traits of cycad leaves, revealing the functional divergence between the Cycadaceae and Zamiaceae families and the differences in trait associations between cycads and angiosperms. The results showed that Zamiaceae species had thicker leaves, higher carbon investments, and greater mechanical resistance compared to Cycadaceae species. Cycad leaves were thicker, tougher, and more tolerant to desiccation compared to angiosperm leaves. The study expands our understanding of the ecological strategies and responses of cycads to climate change.