Journal
ANNALS OF ANATOMY-ANATOMISCHER ANZEIGER
Volume 239, Issue -, Pages -Publisher
ELSEVIER GMBH
DOI: 10.1016/j.aanat.2021.151812
Keywords
Aspirin; Cytokines; Eicosapentaenoic acid; Inflammation; Temporomandibular joint
Categories
Funding
- CNPq (National Board for Scientific and Technological Development) [400957/2016-7]
- SAo Paulo State Research Support Foundation (FAPESP) [2017/17472-3]
Ask authors/readers for more resources
The study showed that omega-3 polyunsaturated fatty acids exhibited anti-inflammatory activity in arthritic rats, and the addition of aspirin increased proteoglycan density but did not affect cytokine expression.
This study aimed to evaluate the effects of omega-3 (w3) polyunsaturated fatty acids, in association with aspirin (AA), on the morphology of cytokine release in the temporomandibular joint (TMJ) of rats induced with rheumatoid arthritis (IR) by injecting 100 mu L of complete Freund's adjuvant with bovine type II collagen at the tail base. Thirty-two adult male rats were divided into treatment groups: Sham, treated with 0.9% sodium chloride (NaCl) p.o.; IR-control, treated with 0.9% NaCl p.o.; IR-w3 treated with w3 PUFAS (85 mg/kg/day p.o.); and IR-w3 + AA treated with w3 (85 mg/kg/day p.o.) + AA (20 mg/kg/day i.p.). After maintained treatment for seven days, the animals were euthanized. Bilateral TMJs from each rat were removed and one was subjected to histological immunoassays and enzyme-linked immunosorbent assays to assess interleukin (IL)-113, tumor necrosis factor-a, and IL-10 levels. Data analysis was performed using the Kruskal-Wallis and Dunn tests. In the IR-w3 and IR-w3 + AA groups, the TMJ was greater than in the IR-control group (P < 0.0001). The addition of AA did not improve the effects of w3 (P = 0.0698). Similarly, the addition of AA conferred no additional effects on the cytokine levels (P > 0.05); however, it increased the proteoglycan density, compared with w3 alone. We found that w3 exhibited anti-inflammatory activity in arthritic rats, and the addition of AA increased proteoglycan density, but did not affect cytokine expression. (c) 2021 Elsevier GmbH. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available