Journal
ANIMAL BIOTECHNOLOGY
Volume -, Issue -, Pages -Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/10495398.2021.1996386
Keywords
KRT26; TCHH; cashmere fineness; milk fat rate; chest width
Funding
- National Natural Science Foundation of China [31802038]
- 69th batch of China Postdoctoral Science Foundation (Regional Special Support Program) [2021M693859]
- Youth seedling project of Liaoning Provincial Department of Education, China [LSNQN201905]
- Liaoning Provincial Department of science and technology, agricultural key issues and industrialization project [2020JH2/10200029]
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This study investigated the relationship between KRT26 and TCHH gene polymorphism and production performance in Liaoning cashmere goats, identifying that these genes were associated with cashmere fineness. The AA and GG genotypes were found to be preferred marker genotypes for cashmere fineness.
Cashmere fineness is getting thicker, which is one of the key problems in cashmere breeding, however, there have been no systematic studies on the molecular regulation of cashmere fineness. The aim of this study was to investigate the relationship between KRT26 and TCHH gene polymorphism and production performance in Liaoning cashmere goats (LCG). The potential single nucleotide polymorphisms (SNPs) of LCG were detected by sequence alignment and PCR-Seq polymorphism of KRT26 and TCHH genes and analyzed the effect of SNPs on production performance by SPSS software. Two SNPs sites (A559T and A6839G) of two genes were detected. The AA genotype of KRT26 A559T locus was the dominant genotype. AG and GG at TCHH A6839G locus were the dominant genotypes. AAAA was the dominant haplotype combination. The results showed that KRT26 and TCHH genes were associated with cashmere fineness of LCG, and A559T (AA) and A6839G (GG) genotypes were the preferred marker genotypes for cashmere fineness, which provided more theoretical basis for further research on cashmere fineness.
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