4.8 Article

Single Cell Imaging of Electrochemiluminescence-Driven Photodynamic Therapy

Journal

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 61, Issue 16, Pages -

Publisher

WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.202117401

Keywords

Electrochemiluminescence; Imaging; Photodynamic Therapy; Single Living Cell

Funding

  1. National Natural Science Foundation [22034003, 21974061, 22074063]

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We report a photodynamic therapy driven by electrochemiluminescence (ECL) with potential clinical applications. The ECL-generated luminescence acts as both optical readout and light source for the excitation of photosensitizer to produce reactive oxygen species (ROS). Real-time monitoring of cellular processes during ECL-PDT was achieved using ECL microscopy with high spatiotemporal resolution.
We report a photodynamic therapy driven by electrochemiluminescence (ECL). The luminescence generated by Ru(bpy)(3)(2+) and co-reactant tripropylamine (TPA) pair acts as both optical readout for ECL imaging, and light source for the excitation of photosensitizer to produce reactive oxygen species (ROS) in photodynamic therapy (PDT) system. The ECL-driven PDT (ECL-PDT) relies on the effective energy transfer from ECL emission to photosensitizer chlorin e6 (Ce6), which sensitizes the surrounding O-2 into ROS. The dynamic process of gradual morphological changes, the variation of cell-matrix adhesions, as well as the increase of cell membrane permeability in the process of ECL-PDT were monitored under ECL microscopy (ECLM) with good spatiotemporal resolution. Combining real-time imaging with ECL-PDT, this new strategy provides not only new insights into dynamic cellular processes, but also promising potential of ECL in clinical applications.

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