Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 60, Issue 43, Pages 23148-23153Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.202109349
Keywords
copper(I); Flim; fluorescence; lifetime; TCSPC
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Funding
- UK Engineering and Physical Sciences Research Council (EPSRC) [EP/L016737/1]
- EPSRC [EP/I003983/1]
- Leverhulme Trust [RPG-2015-345]
- Excellence Fund for Frontier Research, Imperial College London
- EPSRC [EP/I003983/1] Funding Source: UKRI
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A new optical probe based on BODIPY has been developed, which exhibits fluorescence intensity switch-on upon binding to copper(I) and can be used to visualize copper(I) pools in lysosomes of live cells.
Copper is an essential trace element in living organisms with its levels and localisation being carefully managed by the cellular machinery. However, if misregulated, deficiency or excess of copper ions can lead to several diseases. Therefore, it is important to have reliable methods to detect, monitor and visualise this metal in cells. Herein we report a new optical probe based on BODIPY, which shows a switch-on in its fluorescence intensity upon binding to copper(I), but not in the presence of high concentration of other physiologically relevant metal ions. More interestingly, binding to copper(I) leads to significant changes in the fluorescence lifetime of the new probe, which can be used to visualize copper(I) pools in lysosomes of live cells via fluorescence lifetime imaging microscopy (FLIM).
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