Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 61, Issue 6, Pages -Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.202111416
Keywords
Bacterial toxins sensing; Electrochemical single impacts; Lipid membrane; Redox liposomes; Utramicroelectrode
Categories
Funding
- Region Pays de la Loire
- University of Nantes
- French Institute of Sweden
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The detection and diagnosis of the virulence factor Rhamnolipid produced by the pathogen Pseudomonas aeruginosa can be quickly and sensitively achieved using the redox liposome single impact electrochemistry method.
The detection of Rhamnolipid virulence factor produced by Pseudomonas aeruginosa involved in nosocomial infections is reported by using the redox liposome single impact electrochemistry. Redox liposomes based on 1,2-dimyristoyl-sn-glycero-3-phosphocholine as a pure phospholipid and potassium ferrocyanide as an encapsulated redox content are designed for using the interaction of the target toxin with the lipid membrane as a sensing strategy. The electrochemical sensing principle is based on the weakening of the liposomes lipid membrane upon interaction with Rhamnolipid toxin which leads upon impact at an ultramicroelectrode to the breakdown of the liposomes and the release/electrolysis of its encapsulated redox probe. We present as a proof of concept the sensitive and fast sensing of a submicromolar concentration of Rhamnolipid which is detected after less than 30 minutes of incubation with the liposomes, by the appearing of current spikes in the chronoamperometry measurement.
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