Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 61, Issue 10, Pages -Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.202113477
Keywords
Chemical Reaction Network; Dissipative Self-Assembly; DNA Nanoscience; DNAzyme; Transient Catalysis
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Funding
- European Research Council [677960]
- Projekt DEAL
- European Research Council (ERC) [677960] Funding Source: European Research Council (ERC)
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The development of synthetic non-equilibrium systems has opened doors for man-made life-like materials. Through ATP-driven dynamic covalent DNA assembly, we have successfully achieved ATP-fueled transient organization and ATP-fueled DNAzymes for substrate cleavage.
The development of synthetic non-equilibrium systems opens doors for man-made life-like materials. Yet, creating distinct transient functions from artificial fuel-driven structures remains a challenge. Building on our ATP-driven dynamic covalent DNA assembly in an enzymatic reaction network of concurrent ATP-powered ligation and restriction, we introduce ATP-fueled transient organization of functional subunits for various functions. The programmability of the ligation/restriction site allows to precisely organize multiple sticky-end-encoded oligo segments into double-stranded (ds) DNA complexes. We demonstrate principles of ATP-driven organization into sequence-defined oligomers by sensing barcode-embedded targets with different defects. Furthermore, ATP-fueled DNAzymes for substrate cleavage are achieved by transiently ligating two DNAzyme subunits into a dsDNA complex, rendering ATP-fueled transient catalytic function.
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