Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 60, Issue 51, Pages 26555-26560Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.202112574
Keywords
galactosyltransferase; GalNAc-glycosides; general tolerance; UDP-galactosamine
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Funding
- National Institute of General Medical Sciences [U01GM125288, R44GM123820]
- National Institute on Aging [R56AG062258]
- Molecular Basis of Disease Fellowship from Georgia State University
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Accessing structurally diverse glycans is crucial for functional glycomics. Our study demonstrates the general tolerance of galactosyltransferases towards UDP-GalN, enabling the synthesis of novel GalNAc-glycosides. This discovery expands the catalytic capabilities of glycosyltransferases, facilitating the synthesis of diverse glycans and glycoconjugates for biological studies.
Accessing large numbers of structurally diverse glycans and derivatives is essential to functional glycomics. We showed a general tolerance of galactosyltransferases toward uridine-diphosphate-galactosamine (UDP-GalN), which is not a commonly used sugar nucleotide donor. The property was harnessed to develop a two-step chemoenzymatic strategy for facile synthesis of novel and divergent N-acetylgalactosamine (GalNAc)-glycosides and derivatives in preparative scales. The discovery and the application of the new property of existing glycosyltransferases expand their catalytic capabilities in generating novel carbohydrate linkages, thus prompting the synthesis of diverse glycans and glycoconjugates for biological studies.
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