Journal
ANALYTICAL LETTERS
Volume 55, Issue 10, Pages 1566-1576Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/00032719.2021.2016790
Keywords
Thioquinapiperifil; metabolic profile; liquid chromatography quadrupole time of flight; forensic toxicology; high performance liquid chromatography mass spectrometry
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Funding
- Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI) - Ministry of Health & Welfare, Republic of Korea [HI18C2383]
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This study analyzed the metabolites of thioquinapiperifil using LC-Q-TOF-MS and identified multiple metabolites in human and rat samples. The mass error values of all TOF mass results were within 5 ppm, indicating high accuracy. These newly identified metabolites may be useful for evaluating the toxicology of thioquinapiperifil and related forensic cases.
To date, many analogies of sildenafil, tadarafil, and vadenafil, which are phosphodiesterase-5 (PDE5) inhibitor have been reported. On the other hand, a novel type of PDE5 inhibitor, thioquinapiperifil has not yet been evaluated. Therefore, in this study, liquid chromatography quadrupole time of flight mass spectrometry (LC-Q-TOF-MS) was conducted to determine the in vitro and in vivo metabolites of thioquinapiperifil. Metabolites in urine and feces samples of rats injected with thioquinapiperifil and in human liver microsomal extract were characterized to examine its possible metabolic pathways. Metabolized samples were extracted and precipitated using acetonitrile and subsequently injected into the LC-Q-TOF-MS system. Peaks of interest were analyzed via tandem-mass spectrometry to identify the metabolite structure. A total of 11, 5, and 7 metabolites were detected in human liver microsomal extract, rat urine, and rat feces, respectively. The mass error values of all TOF mass results were within 5 ppm, indicating high accuracy. These newly identified metabolites may be useful for evaluating the toxicology of thioquinapiperifil and this knowledge may be applied to related forensic cases.
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