4.8 Article

Programming the trans-cleavage Activity of CRISPR-Cas13a by Single-Strand DNA Blocker and Its Biosensing Application

Related references

Note: Only part of the references are listed.
Article Chemistry, Multidisciplinary

An Ultralocalized Cas13a Assay Enables Universal and Nucleic Acid Amplification-Free Single-Molecule RNA Diagnostics

Tian Tian et al.

Summary: The confinement effect-inspired Cas13a assay for single-molecule RNA diagnostics eliminates the need for nucleic acid amplification and reverse transcription, achieving a >10,000-fold enhancement in sensitivity and enabling absolute digital single-molecule RNA quantitation. Its broad applicability and accurate detection of various RNA molecules at the single-molecule level make it a potential dominant rival to RT-qPCR.

ACS NANO (2021)

Article Biochemistry & Molecular Biology

Amplification-free detection of SARS-CoV-2 with CRISPR-Cas13a and mobile phone microscopy

Parinaz Fozouni et al.

Summary: This study developed an amplification-free CRISPR-Cas13a assay for direct detection of SARS-CoV-2, showing rapid, highly sensitive, and mobile phone-based readout capabilities.
Article Biochemistry & Molecular Biology

Sequence-independent RNA sensing and DNA targeting by a split domain CRISPR-Cas12a gRNA switch

Scott P. Collins et al.

Summary: The modular design of Cas12a gRNA switches was demonstrated, decoupling the selection restriction of trigger and guide sequences, allowing them to respond to different triggers and target different DNA sequences. This design enables Cas12a targeting activity to be dependent on cellular metabolism and stress, further connecting CRISPR technologies with cellular states.

NUCLEIC ACIDS RESEARCH (2021)

Review Chemistry, Multidisciplinary

CRISPR Systems for COVID-19 Diagnosis

Hossein Rahimi et al.

Summary: The emergence of COVID-19 in 2019 has become a global pandemic, leading to serious concerns worldwide. The development of CRISPR-based diagnostic systems has reshaped molecular diagnosis and inspired researchers to develop more efficient methods for COVID-19 detection and treatment. The advantages of CRISPR-based COVID-19 diagnostic systems include high detection speed, sensitivity, precision, portability, and the elimination of the need for specialized laboratory equipment.

ACS SENSORS (2021)

Article Multidisciplinary Sciences

A CRISPR-Cas autocatalysis-driven feedback amplification network for supersensitive DNA diagnostics

Kai Shi et al.

Summary: CRISPR-Cas-only amplification network (CONAN) utilizes a Cas12a-driven reaction network to construct a positive feedback circuit, enabling isothermally amplified detection of genomic DNA with attomolar sensitivity, showcasing its potential as a powerful tool for disease diagnostics.

SCIENCE ADVANCES (2021)

Article Multidisciplinary Sciences

Conditional control of RNA-guided nucleic acid cleavage and gene editing

Shao-Ru Wang et al.

NATURE COMMUNICATIONS (2020)

Article Chemistry, Multidisciplinary

Spatiotemporal Control of CRISPR/Cas9 Function in Cells and Zebrafish using Light-Activated Guide RNA

Wenyuan Zhou et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2020)

Article Chemistry, Multidisciplinary

Photoswitchable gRNAs for Spatiotemporally Controlled CRISPR-Cas-Based Genomic Regulation

Elena Moroz-Omori et al.

ACS CENTRAL SCIENCE (2020)

Review Chemistry, Multidisciplinary

CRISPR Mediated Biosensing Toward Understanding Cellular Biology and Point-of-Care Diagnosis

Yifan Dai et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2020)

Review Biochemistry & Molecular Biology

Allosteric regulation of CRISPR-Cas9 for DNA-targeting and cleavage

Zhicheng Zuo et al.

CURRENT OPINION IN STRUCTURAL BIOLOGY (2020)

Article Multidisciplinary Sciences

Very fast CRISPR on demand

Yang Liu et al.

SCIENCE (2020)

Review Biochemistry & Molecular Biology

Molecular Mechanisms of RNA Targeting by Cas13-containing Type VI CRISPR-Cas Systems

Mitchell R. O'Connell

JOURNAL OF MOLECULAR BIOLOGY (2019)

Review Biotechnology & Applied Microbiology

Advances in CRISPR-Cas systems for RNA targeting, tracking and editing

Fei Wang et al.

BIOTECHNOLOGY ADVANCES (2019)

Article Biochemistry & Molecular Biology

Enhanced Cas12a editing in mammalian cells and zebrafish

Pengpeng Liu et al.

NUCLEIC ACIDS RESEARCH (2019)

Article Multidisciplinary Sciences

Two HEPN domains dictate CRISPR RNA maturation and target cleavage in Cas13d

Bo Zhang et al.

NATURE COMMUNICATIONS (2019)

Article Multidisciplinary Sciences

Controlling CRISPR-Cas9 with ligand-activated and ligand-deactivated sgRNAs

Kale Kundert et al.

NATURE COMMUNICATIONS (2019)

Article Multidisciplinary Sciences

Switching the activity of Cas12a using guide RNA strand displacement circuits

Lukas Oesinghaus et al.

NATURE COMMUNICATIONS (2019)

Article Biochemistry & Molecular Biology

Mechanistic Insights into the cis- and trans-Acting DNase Activities of Cas12a

Daan C. Swarts et al.

MOLECULAR CELL (2019)

Article Biochemistry & Molecular Biology

Riboregulated toehold-gated gRNA for programmable CRISPR-Cas9 function

Ka-Hei Siu et al.

NATURE CHEMICAL BIOLOGY (2019)

Review Biotechnology & Applied Microbiology

CRISPR/Cas Systems towards Next-Generation Biosensing

Yi Li et al.

TRENDS IN BIOTECHNOLOGY (2019)

Article Biochemistry & Molecular Biology

Genome editing by natural and engineered CRISPR-associated nucleases

Wen Y. Wu et al.

NATURE CHEMICAL BIOLOGY (2018)

Article Multidisciplinary Sciences

CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity

Janice S. Chen et al.

SCIENCE (2018)

Article Multidisciplinary Sciences

Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6

Jonathan S. Gootenberg et al.

SCIENCE (2018)

Letter Cell Biology

CRISPR-Cas12a-assisted nucleic acid detection

Shi-Yuan Li et al.

CELL DISCOVERY (2018)

Article Biochemistry & Molecular Biology

Structural Basis for the RNA-Guided Ribonuclease Activity of CRISPR-Cas13d

Cheng Zhang et al.

Article Biochemistry & Molecular Biology

RNA Guide Complementarity Prevents Self-Targeting in Type VI CRISPR Systems

Alexander J. Meeske et al.

MOLECULAR CELL (2018)

Review Chemistry, Analytical

Emerging Approaches for Spatiotemporal Control of Targeted Genome with Inducible CRISPR-Cas9

Yuta Nihongaki et al.

ANALYTICAL CHEMISTRY (2018)

Article Biotechnology & Applied Microbiology

Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array

Bernd Zetsche et al.

NATURE BIOTECHNOLOGY (2017)

Article Biochemistry & Molecular Biology

Multidimensional chemical control of CRISPR-Cas9

Basudeb Maji et al.

NATURE CHEMICAL BIOLOGY (2017)

Article Biochemistry & Molecular Biology

RNA Targeting by Functionally Orthogonal Type VI-A CRISPR-Cas Enzymes

Alexandra East-Seletsky et al.

MOLECULAR CELL (2017)

Article Multidisciplinary Sciences

RNA targeting with CRISPR-Cas13

Omar O. Abudayyeh et al.

NATURE (2017)

Article Biochemistry & Molecular Biology

Guide-bound structures of an RNA-targeting A-cleaving CRISPR-Cas13a enzyme

Gavin J. Knott et al.

NATURE STRUCTURAL & MOLECULAR BIOLOGY (2017)

Article Multidisciplinary Sciences

Nucleic acid detection with CRISPR-Cas13a/C2c2

Jonathan S. Gootenberg et al.

SCIENCE (2017)

Article Multidisciplinary Sciences

Aptazyme-embedded guide RNAs enable ligand-responsive genome editing and transcriptional activation

Weixin Tang et al.

NATURE COMMUNICATIONS (2017)

Article Multidisciplinary Sciences

Rational design of inducible CRISPR guide RNAs for de novo assembly of transcriptional programs

Quentin R. V. Ferry et al.

NATURE COMMUNICATIONS (2017)

Article Biochemistry & Molecular Biology

Guide-bound structures of an RNA-targeting A-cleaving CRISPR-Cas13a enzyme

Gavin J. Knott et al.

NATURE STRUCTURAL & MOLECULAR BIOLOGY (2017)

Review Biochemical Research Methods

Switchable Cas9

Florian Richter et al.

CURRENT OPINION IN BIOTECHNOLOGY (2017)

Article Biochemistry & Molecular Biology

The Molecular Architecture for RNA-Guided RNA Cleavage by Cas13a

Liang Liu et al.

Article Biochemistry & Molecular Biology

Two Distant Catalytic Sites Are Responsible for C2c2 RNase Activities

Liang Liu et al.

Article Multidisciplinary Sciences

Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection

Alexandra East-Seletsky et al.

NATURE (2016)

Article Biotechnology & Applied Microbiology

Profiling of engineering hotspots identifies an allosteric CRISPR-Cas9 switch

Benjamin L. Oakes et al.

NATURE BIOTECHNOLOGY (2016)

Article Biochemistry & Molecular Biology

A chemical-inducible CRISPR-Cas9 system for rapid control of genome editing

Kaiwen Ivy Liu et al.

NATURE CHEMICAL BIOLOGY (2016)

Article Chemistry, Multidisciplinary

Small-molecule control of protein function through Staudinger reduction

Ji Luo et al.

NATURE CHEMISTRY (2016)

Article Multidisciplinary Sciences

C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector

Omar O. Abudayyeh et al.

SCIENCE (2016)

Article Chemistry, Multidisciplinary

Optical Control of CRISPR/Cas9 Gene Editing

James Hemphill et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2015)

Article Biotechnology & Applied Microbiology

Photoactivatable CRISPR-Cas9 for optogenetic genome editing

Yuta Nihongaki et al.

NATURE BIOTECHNOLOGY (2015)

Article Biochemistry & Molecular Biology

Small molecule-triggered Cas9 protein with improved genome-editing specificity

Kevin M. Davis et al.

NATURE CHEMICAL BIOLOGY (2015)

Article Chemistry, Multidisciplinary

Selective, rapid and optically switchable regulation of protein function in live mammalian cells

Yu-Hsuan Tsai et al.

NATURE CHEMISTRY (2015)

Article Biochemistry & Molecular Biology

Development of an intein-mediated split-Cas9 system for gene therapy

Dong-Jiunn Jeffery Truong et al.

NUCLEIC ACIDS RESEARCH (2015)

Article Chemistry, Multidisciplinary

Palladium-triggered deprotection chemistry for protein activation in living cells

Jie Li et al.

NATURE CHEMISTRY (2014)

Article Multidisciplinary Sciences

RNA-Guided Human Genome Engineering via Cas9

Prashant Mali et al.

SCIENCE (2013)