4.8 Article

Enhanced Top-Down Protein Characterization with Electron Capture Dissociation and Cyclic Ion Mobility Spectrometry

Related references

Note: Only part of the references are listed.
Article Chemistry, Analytical

Anomeric Retention of Carbohydrates in Multistage Cyclic Ion Mobility (IMSn): De Novo Structural Elucidation of Enzymatically Produced Mannosides

Simon Ollivier et al.

Summary: Carbohydrates are complex structures with different levels of isomerism, notably the anomerism of the glycosidic bond, which are still challenging analysts today. Recent research has shown that anomerism can be preserved upon gas-phase fragmentation and high-resolution ion mobility (IMS) can distinguish anomers. The study demonstrates that high-resolution IMS and IMSn can be used to characterize the intrachain anomerism in tri- and tetrasaccharides in a biological medium, providing the first evidence that a single carbohydrate-active enzyme can synthesize both alpha- and beta-glycosidic linkages.

ANALYTICAL CHEMISTRY (2021)

Article Biochemical Research Methods

Cyclic Ion Mobility-Collision Activation Experiments Elucidate Protein Behavior in the Gas Phase

Charles Eldrid et al.

Summary: Ion mobility coupled to mass spectrometry (IM-MS) is widely utilized to study protein dynamics and structure in the gas phase. A high-resolution cyclic IM-mass spectrometer (cIM-MS) allows for multiple rounds of tandem IM experiments to be conducted, providing insights into protein unfolding pathways and dynamics. The IMn-MS technique shows evidence of interconversion between compact and extended protein structures, and suggests certain conformations have low energy barriers between them.

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY (2021)

Article Biochemical Research Methods

Sequential Ion-Ion Reactions for Enhanced Gas-Phase Sequencing of Large Intact Proteins in a Tribrid Orbitrap Mass Spectrometer

Jake T. Kline et al.

Summary: Obtaining extensive sequencing of intact proteins is crucial for determining different proteoforms arising from the same gene. Advanced ion dissociation techniques, such as electron transfer dissociation and ultraviolet photodissociation, can improve sequencing results but have limitations for proteins >= 30 kDa. Proton transfer charge reduction (PTCR) combined with other ion activation techniques can enhance sequencing coverage for large proteins, addressing issues of signal overlap and low signal-to-noise fragments in mass spectrometry analysis.

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY (2021)

Article Chemistry, Analytical

Advanced Strategies for Proton-Transfer Reactions Coupled with Parallel Ion Parking on a 21 T FT-ICR MS for Intact Protein Analysis

Chad R. Weisbrod et al.

Summary: PTR combined with PIP technology can significantly improve the spectral S/N of intact proteins and accelerate mass spectrometry analysis. Rapid park can increase reaction rates by 5-10x and improve tandem MS sequencing speed by reducing ion injection time.

ANALYTICAL CHEMISTRY (2021)

Article Chemistry, Analytical

Toward Sequencing the Human Milk Glycome: High-Resolution Cyclic Ion Mobility Separations of Core Human Milk Oligosaccharide Building Blocks

Tyler L. Peterson et al.

Summary: Human milk oligosaccharides (HMOs) play a crucial role in promoting the healthy development of the brain-gut axes in infants and are tailored for each infant based on various biological factors. The complex structures of HMOs lead to a high degree of isomeric heterogeneity, making accurate characterization challenging. New analytical methods are needed to better understand the biological roles of HMOs and potentially incorporate them into infant formula.

ANALYTICAL CHEMISTRY (2021)

Review Chemistry, Analytical

Ion Activation Methods for Peptides and Proteins

Luis A. Macias et al.

ANALYTICAL CHEMISTRY (2020)

Article Biochemical Research Methods

High-resolution ion mobility spectrometry-mass spectrometry of isomeric/isobaric ribonucleotide variants

Thomas Kenderdine et al.

JOURNAL OF MASS SPECTROMETRY (2020)

Article Biochemical Research Methods

Application of a novel cyclic ion mobility-mass spectrometer to the analysis of synthetic polymers: A preliminary evaluation

Eleanor Riches et al.

RAPID COMMUNICATIONS IN MASS SPECTROMETRY (2020)

Article Chemistry, Analytical

LESA Cyclic Ion Mobility Mass Spectrometry of Intact Proteins from Thin Tissue Sections

Emma K. Sisley et al.

ANALYTICAL CHEMISTRY (2020)

Article Chemistry, Analytical

A Cyclic Ion Mobility-Mass Spectrometry System

Kevin Giles et al.

ANALYTICAL CHEMISTRY (2019)

Article Biochemical Research Methods

Cyclic Ion Mobility Mass Spectrometry Distinguishes Anomers and Open-Ring Forms of Pentasaccharides

Jakub Ujma et al.

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY (2019)

Article Chemistry, Analytical

Gas Phase Stability of Protein Ions in a Cyclic Ion Mobility Spectrometry Traveling Wave Device

Charles Eldrid et al.

ANALYTICAL CHEMISTRY (2019)

Review Chemistry, Analytical

Historical, current and future developments of travelling wave ion mobility mass spectrometry: A personal perspective

Iain D. G. Campuzano et al.

TRAC-TRENDS IN ANALYTICAL CHEMISTRY (2019)

Article Chemistry, Analytical

Proton Transfer Charge Reduction Enables High-Throughput Top-Down Analysis of Large Proteoforms

Romain Huguet et al.

ANALYTICAL CHEMISTRY (2019)

Article Biochemical Research Methods

Sequencing Larger Intact Proteins (30-70 kDa) with Activated Ion Electron Transfer Dissociation

Nicholas M. Riley et al.

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY (2018)

Article Biochemical Research Methods

Multiple Gas-Phase Conformations of a Synthetic Linear Poly(acrylamide) Polymer Observed Using Ion Mobility-Mass Spectrometry

Jean R. N. Haler et al.

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY (2017)

Article Biochemical Research Methods

Front-End Electron Transfer Dissociation Coupled to a 21 Tesla FT-ICR Mass Spectrometer for Intact Protein Sequence Analysis

Chad R. Weisbrod et al.

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY (2017)

Article Chemistry, Analytical

Characterization and Identification of Dityrosine Cross-Linked Peptides Using Tandem Mass Spectrometry

Soumya Mukherjee et al.

ANALYTICAL CHEMISTRY (2017)

Review Chemistry, Analytical

Ion Mobility-Mass Spectrometry: Time-Dispersive Instrumentation

Jody C. May et al.

ANALYTICAL CHEMISTRY (2015)

Article Biochemical Research Methods

Characterization of top-down ETD in a travelling-wave ion guide

Frederik Lermyte et al.

METHODS (2015)

Article Chemistry, Analytical

High Resolution Trapped Ion Mobility Spectrometery of Peptides

Joshua A. Silveira et al.

ANALYTICAL CHEMISTRY (2014)

Article Biochemical Research Methods

ECD of Tyrosine Phosphorylation in a Triple Quadrupole Mass Spectrometer with a Radio-Frequency-Free Electromagnetostatic Cell

Valery G. Voinov et al.

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY (2014)

Review Biochemical Research Methods

Top-down proteomics in health and disease: Challenges and opportunities

Zachery R. Gregorich et al.

PROTEOMICS (2014)

Article Chemistry, Analytical

Autophaser: An Algorithm for Automated Generation of Absorption Mode Spectra for FT-ICR MS

David P. A. Kilgour et al.

ANALYTICAL CHEMISTRY (2013)

Article Chemistry, Multidisciplinary

Complete Protein Characterization Using Top-Down Mass Spectrometry and Ultraviolet Photodissociation

Jared B. Shaw et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2013)

Letter Biochemical Research Methods

Proteoform: a single term describing protein complexity

Lloyd M. Smith et al.

NATURE METHODS (2013)

Review Chemistry, Analytical

Advancements in Top-Down Proteomics

Hu Zhou et al.

ANALYTICAL CHEMISTRY (2012)

Article Chemistry, Multidisciplinary

Bound Cations Significantly Stabilize the Structure of Multiprotein Complexes in the Gas Phase

Linjie Han et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2012)

Article Physics, Atomic, Molecular & Chemical

A conceptual approach for FT-ICR cell harmonization utilizing external shim electrodes

Aleksey V. Tolmachev et al.

INTERNATIONAL JOURNAL OF MASS SPECTROMETRY (2012)

Article Chemistry, Analytical

On the Scalability and Requirements of Whole Protein Mass Spectrometry

Philip D. Compton et al.

ANALYTICAL CHEMISTRY (2011)

Article Biochemical Research Methods

Initial Experimental Characterization of a New Ultra-High Resolution FTICR Cell with Dynamic Harmonization

Eugene N. Nikolaev et al.

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY (2011)

Article Chemistry, Analytical

A Scanning Frequency Mode for Ion Cyclotron Mobility Spectrometry

Rebecca S. Glaskin et al.

ANALYTICAL CHEMISTRY (2010)

Article Chemistry, Analytical

Radio-Frequency-Free Cell for Electron Capture Dissociation in Tandem Mass Spectrometry

Valery G. Voinov et al.

ANALYTICAL CHEMISTRY (2009)

Article Chemistry, Analytical

High-Resolution Ion Cyclotron Mobility Spectrometry

Samuel I. Merenbloom et al.

ANALYTICAL CHEMISTRY (2009)

Article Biochemical Research Methods

Performance Evaluation of a High-field Orbitrap Mass Analyzer

Alexander Makarov et al.

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY (2009)

Letter Biochemical Research Methods

Electron capture dissociation in a linear radiofrequency-free magnetic cell

Valery G. Voinov et al.

RAPID COMMUNICATIONS IN MASS SPECTROMETRY (2008)

Article Physics, Atomic, Molecular & Chemical

An investigation of the mobility separation of some peptide and protein ions using a new hybrid quadrupole/travelling wave IMS/oa-ToF instrument

Steven D. Pringle et al.

INTERNATIONAL JOURNAL OF MASS SPECTROMETRY (2007)

Review Biotechnology & Applied Microbiology

Post-translational modifications in the context of therapeutic proteins

Gary Walsh et al.

NATURE BIOTECHNOLOGY (2006)

Article Multidisciplinary Sciences

Peptide and protein sequence analysis by electron transfer dissociation mass spectrometry

JEP Syka et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2004)