Journal
ANALYTICAL CHEMISTRY
Volume 93, Issue 49, Pages 16350-16359Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.1c02368
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Funding
- Botnar Research Centre for Child Health as part of the Fast Track Call for Acute Global Health Challenges
- BRIDGE program by the Swiss National Science Foundation
- Innosuisse
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This study aimed to develop a rapid diagnostic approach for identifying gene mutations associated with SARS-CoV-2 VOCs, which can be run on various laboratory equipment. The assays showed excellent diagnostic performance and are suitable for resource-limited settings.
The need for tools that facilitate rapid detection and continuous monitoring of SARS-CoV-2 variants of concern (VOCs) is greater than ever, as these variants are more transmissible and therefore increase the pressure of COVID-19 on healthcare systems. To address this demand, we aimed at developing and evaluating a robust and fast diagnostic approach for the identification of SARSCoV-2 VOC-associated spike gene mutations. Our diagnostic assays detect the E484K and N501Y single-nucleotide polymorphisms (SNPs) as well as a spike gene deletion (HV69/70) and can be run on standard laboratory equipment or on the portable rapid diagnostic technology platform peakPCR. The assays achieved excellent diagnostic performance when tested with RNA extracted from culture-derived SARS-CoV-2 VOC lineages and clinical samples collected in Equatorial Guinea, Central-West Africa. Simplicity of usage and the relatively low cost are advantages that make our approach well suitable for decentralized and rapid testing, especially in resource-limited settings.
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