Journal
ANALYTICAL CHEMISTRY
Volume 93, Issue 42, Pages 14334-14342Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.1c03636
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Funding
- National Natural Science Foundation of China [21874095]
- China Postdoctoral Science Foundation [2020M683538]
- Natural Science Foundation of Shaanxi Province, China [2020JM-434, 2021JQ-437]
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The SEIA system, based on self-extending DNA-mediated isothermal amplification, provides a simple and efficient way to achieve rapid, robust, and significant signal amplification, demonstrating potential for broad-spectrum target detection in various scenarios.
Signal amplification is critical to achieving sensitive biosensing, but complex strategies often bring problems like system instability, false positive, or narrow target spectrum. Here, a self-extending DNA-mediated isothermal amplification (SEIA) system with simple reaction components is introduced to achieve rapid, robust, and significant signal amplification. In SEIA, based on spontaneous refolding of specific DNA domains and using the previous generation product as a template, a DNA strand can extend continuously in an approximate exponential growth pattern, which was accurately predicted by our formula and well supported by AFM results. Based on a set of proof-of-concept experiments, it was proved that the SEIA system can output different signals and flexibly integrate various functional nucleic acids, which makes it suitable for different scenarios and realizes broad-spectrum target detection. Taking into account the advantages of simplicity, flexibility, and efficiency, the SEIA system as an independent signal amplification module will enrich the toolbox of biosensing design.
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