Journal
ANALYTICAL CHEMISTRY
Volume 94, Issue 6, Pages 2827-2834Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.1c04506
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Funding
- National Natural Science Foundation of China [21575088, 31800670]
- Natural Science Foundation of Shanghai [19ZR1474200]
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A system utilizing magnetism to reversibly control the activity of DNAzyme has been developed, allowing for easy switching between an OFF and ON state. This system provides a reusable platform for researchers to explore the regulation of conformation-dependent polymer activity.
Controllable regulation of enzyme activity is an important prerequisite for the in-depth application of enzymes, especially in today's intelligent era. However, irreversible regulation and cumbersome operation make this goal difficult to achieve. Here, by adopting magnetism and a harmless, noncontact, and time- and space-controllable physical element, we developed a system that could conveniently and reversibly regulate the activity of DNAzyme. In this system, the strands of the DNAzyme could be stretched or folded by applying or removing a magnetic field. Thereby, the conformation-dependent endonuclease activity of the DNAzyme could be facilely switched between an OFF and ON state. This system provides a reusable platform for the control of enzyme catalytic activity through magnetism, which provides guidance for further application in some related scientific research, especially the regulation of the activity of conformation-dependent polymers (DNAzymes, aptamers, and peptides).
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