4.8 Article

Low-Cost, User-Friendly, All-Integrated Smartphone-Based Microplate Reader for Optical-Based Biological and Chemical Analyses

Journal

ANALYTICAL CHEMISTRY
Volume 94, Issue 2, Pages 1271-1285

Publisher

AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.1c04491

Keywords

-

Funding

  1. CERCA programme, Generalitat de Catalunya
  2. MCIN/AEI [SEV-2017-0706, MAT2017-87202-P]
  3. Consejo Superior de Investigaciones Cientificas (CSIC)
  4. European Union [689341, 881603]
  5. FEDER Una manera de hacer Europa
  6. European Research Council [754510]

Ask authors/readers for more resources

The quantitative detection of different molecular targets is crucial for various activities. This study presents a smartphone-based device that enables high-precision laboratory analysis directly at the point of care.
The quantitative detection of different molecular targets is of utmost importance for a variety of human activities, ranging from healthcare to environmental studies. Bioanalytical methods have been developed to solve this and to achieve the quantification of multiple targets from small volume samples. Generally, they can be divided into two different classes: point of care (PoC) and laboratory-based approaches. The former is rapid, low-cost, and user-friendly; however, the majority of the tests are semiquantitative, lacking in specificity and sensitivity. On the contrary, laboratory-based approaches provide high sensitivity and specificity, but the bulkiness of experimental instruments and complicated protocols hamper their use in resource-limited settings. In response, here we propose a smartphone-based device able to support laboratory-based optical techniques directly at the point of care. Specifically, we designed and fabricated a portable microplate reader that supports colorimetric, fluorescence, luminescence, and turbidity analyses. To demonstrate the potential of the device, we characterized its analytical performance by detecting a variety of relevant molecular targets (ranging from antibodies, toxins, drugs, and classic fluorophore dyes) and we showed how the estimated results are comparable to those obtained from a commercial microplate reader. Thanks to its low cost (<$300), portability (27 cm [length] x 18 cm [width] x 7 cm [height]), commercially available components, and open-source-based system, we believe it represents a valid approach to bring high-precision laboratory-based analysis at the point of care.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.8
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available