Journal
ANALYTICAL CHEMISTRY
Volume 93, Issue 44, Pages 14730-14736Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.1c03190
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Funding
- European Research Council [788697-GLYCANAL]
- Swiss National Science Foundation [206021_177004]
- Swiss National Science Foundation (SNF) [206021_177004] Funding Source: Swiss National Science Foundation (SNF)
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The study demonstrates a new approach using infrared spectroscopy for identifying isomers in a glycan mixture, providing a solution for identifying isomeric components in a mixture when they cannot be separated by high-resolution ion mobility spectrometry.
The analysis of glycans presents a significant challenge that arises from their isomeric heterogeneity. While high-resolution ion mobility spectrometry (IMS) has shown the ability to resolve subtly different glycan isomers, their unambiguous assignment remains difficult. Here, we demonstrate an infrared (IR) spectroscopic approach for identifying isomers in a glycan mixture. To display the feasibility of this approach, we have constructed a small database of cryogenic spectra of five lacto-N-fucopentaose (LNFP) and six disaccharide isomers and demonstrated that in the cases where they cannot be separated by IMS, we can use a cryogenic IR spectrum to identify the isomeric components of a mixture.
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