A Bio-inspired Extended-Gate Metal-Oxide-Semiconductor Field-Effect-Transistor for Highly Sensitive Amino Acid Enantiodiscrimination

As the most important small molecules revealing the origins of life, amino acids (AAs) play essential roles in living organisms and their facile enantiodiscrimination has long been a great challenge for analytical chemists. Inspired by the specific stereomatching effect between biomolecules and AA enantiomers, herein, we first developed a bio-inspired highly sensitive platform based on an extended-gate metal-oxide-semiconductor field-effect-transistor (EG-MOSFET) for highly sensitive AA enantiodiscrimination. Bovine serum albumin (BSA) was self-assembled on deposited Au surfaces to afford the extended gate (EG) sensing unit, and its enantiorecognition ability was initially verified using common electrochemical techniques. The EG was thereafter installed to a MOSFET to build the desired BSA-EG-MOSFET highly sensitive chiral sensing platform, which realized the efficient enantiodiscrimination of essential AAs with high sensitivity, where effective chiral resolution was achieved at the femtomole level to phenylalanine (Phe). Combining molecular docking and circular dichroism spectroscopy, the weak intermolecular interactions between BSA and AAs enantiomers were investigated and the mechanism for signal amplification was proposed. Our results demonstrate that the as-fabricated biosensor has great potential in highly sensitive chiral sensing fields and can also afford a potential tool for biomolecular interaction investigations.

Automated summary

In this study, a bio-inspired highly sensitive platform based on EG-MOSFET was developed for efficient enantiodiscrimination of essential amino acids. The platform successfully achieved high sensitivity chiral resolution at the femtomole level to phenylalanine, showing great potential in chiral sensing fields and biomolecular interaction investigations.