Journal
ANALYTICAL BIOCHEMISTRY
Volume 631, Issue -, Pages -Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2021.114368
Keywords
Plantaricin LD1; Nisin; Colorimetric response; FTIR; SEM; PDA Vesicles
Funding
- Department of Biotechnology (DBT) [BT/PR8911/NDB/39/423/2013]
- Indian Council of Medical Research (ICMR) [5/9/1117/2013-NUT]
- New Delhi, India
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The study synthesized polydiacetylene (PDA) vesicles as artificial membrane biosensors for detecting plantaricin LD1, showing potential application in detecting bacteriocins with colorimetric response and infrared absorbance changes.
The interaction of antimicrobial peptides with membrane lipids plays a major role in numerous physiological processes. In this study, polydiacetylene (PDA) vesicles were synthesized using 10, 12-tricosadiynoic acid (TRCDA) and 1, 2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC). These vesicles were applied as artificial membrane biosensor for the detection of plantaricin LD1 purified from Lactobacillus plantarum LD1. Plantaricin LD1 (200 mu g/mL) was able to interact with PDA vesicles by changing the color from blue to red with colorimetric response 30.26 +/- 0.59. Nisin (200 mu g/mL), used as control, also changed the color of the vesicles with CR% 50.56 +/- 0.98 validating the assay. The vesicles treated with nisin and plantaricin LD1 showed increased infrared absorbance at 1411.46 and 1000-1150 cm(-1) indicated the interaction of bacteriocins with phospholipids and fatty acids, respectively suggesting membrane-acting nature of these bacteriocins. Further, microscopic observation of bacteriocin-treated vesicles showed several damages indicating the interaction of bacteriocins. These findings suggest that the PDA vesicles may be used as bio-mimetic sensor for the detection of bacteriocins produced by several probiotics in food and therapeutic applications.
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